Cloning and sequence analysis of the dhaT gene of the 1,3-propanediol regulon from Klebsiella pneumoniae

被引：0
作者：
Zheng, YY [1 ]
Cao, Y [1 ]
Fang, BS [1 ]
机构：
[1] Huaqiao Univ, Dept Bioengn & Biotechnol, Quanzhou 362011, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 03期
关键词：
homology modeling; Klebsiella pneumoniae; 1,3-propanediol oxidoreductase; sequence analysis;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
1,3-Propanediol oxidoreductase encoded by dhaT gene, a gene of 1,3-propanediol regulon, is important in converting glycerol to 1,3-propanediol in Klebsiella pneumoniae. DhaT gene was amplified from the genome of K. pneumoniae, sequenced and its amino acid sequence deduced. A predicted secondary structure and 3D-structural model was constructed by homology modelling. Based on these results, we infer that 1,3-propanediol oxidoreductase belongs to NAD(P)-dependent alcohol dehydrogenase group III of iron-activated dehydrogenases.
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页码：251 / 255
页数：5
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