Immunological characterization of Neospora caninum cyclophilin

被引：12
作者：
Kameyama, Kyohko [1 ]
Nishimura, Maki [1 ]
Punsantsogvoo, Myagmarsuren [1 ]
Ibrahim, Hany M. [1 ]
Xuan, Xuenan [1 ]
Furuoka, Hidefumi [2 ]
Nishikawa, Yoshifumi [1 ]
机构：
[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan
[2] Obihiro Univ Agr & Vet Med, Div Pathobiol Sci, Dept Basic Vet Med, Obihiro, Hokkaido 0808555, Japan
来源：
PARASITOLOGY | 2012年 / 139卷 / 03期
基金：
日本学术振兴会;
关键词：
Neospora caninum; cyclophilin; cell migration; CD4(+) T-CELLS; TOXOPLASMA-GONDII; GAMMA-INTERFERON; HOST-CELLS; INFECTION; PROTEIN; IDENTIFICATION; PROLIFERATION; FIBROBLASTS; MACROPHAGES;
D O I：
10.1017/S0031182011002022
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Neospora caninum is an intracellular parasite that poses a unique ability to infect a variety of cell types by causing host cell migration. Although previous studies demonstrated that parasite-derived proteins could trigger host cell migration, the related molecules have yet to be determined. Our study aimed to investigate the relationship between Neospora-derived molecules and host cell migration using recombinant protein of N. caninum cyclophilin (NcCyp). Indirect fluorescent antibody test revealed that NcCyp was expressed in the tachyzoite cytosol. Furthermore, NcCyp release from extracellular parasites was detected by sandwich enzyme-linked immunosorbent assay in a time-dependent manner. Recombinant NcCyp caused the cysteine-cysteine chemokine receptor 5-dependent migration of murine and bovine cells. Furthermore, immunohistochemistry indicated that NcCyp was consistently detected in tachyzoites distributed within or around the brain lesions. In conclusion, N. caninum-derived cyclophilin appears to contribute to host cell migration, thereby maintaining parasite/host interactions.
引用
收藏
页码：294 / 301
页数：8
相关论文
共 26 条
  • [1] Molecular mimicry of a CCR5 binding-domain in the microbial activation of dendritic cells
    Alberti, J
    Valenzuela, JG
    Carruthers, VB
    Hieny, S
    Andersen, J
    Charest, H
    Sousa, CRE
    Fairlamb, A
    Ribeiro, JM
    Sher, A
    [J]. NATURE IMMUNOLOGY, 2003, 4 (05) : 485 - 490
  • [2] Interferon-γ and interleukin-12 mediate protection to acute Neospora caninum infection in BALB/c mice
    Baszler, TV
    Long, MT
    McElwain, TF
    Mathison, BA
    [J]. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1999, 29 (10) : 1635 - 1646
  • [3] THE NEUROPATHOLOGY IN TOXOPLASMOSIS-LIKE INFECTION CAUSED BY A NEWLY RECOGNIZED CYST-FORMING SPOROZOON IN DOGS
    BJERKAS, I
    PRESTHUS, J
    [J]. APMIS, 1989, 97 (05) : 459 - 468
  • [4] The protozoan Neospora caninum directly triggers bovine NK cells to produce gamma interferon and to kill infected fibroblasts
    Boysen, P
    Klevar, S
    Olsen, I
    Storset, AK
    [J]. INFECTION AND IMMUNITY, 2006, 74 (02) : 953 - 960
  • [5] NEOSPOROSIS
    DUBEY, JP
    LINDSAY, DS
    [J]. PARASITOLOGY TODAY, 1993, 9 (12): : 452 - 458
  • [6] Molecular and immunodiagnostic investigations on bovine neosporosis in Switzerland
    Gottstein, B
    Hentrich, B
    Wyss, R
    Thur, B
    Busato, A
    Stark, KDC
    Muller, N
    [J]. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1998, 28 (04) : 679 - 691
  • [7] Immunophilins: Beyond immunosuppression
    Hamilton, GS
    Steiner, JP
    [J]. JOURNAL OF MEDICINAL CHEMISTRY, 1998, 41 (26) : 5119 - 5143
  • [8] CYCLOPHILIN - A SPECIFIC CYTOSOLIC BINDING-PROTEIN FOR CYCLOSPORIN-A
    HANDSCHUMACHER, RE
    HARDING, MW
    RICE, J
    DRUGGE, RJ
    [J]. SCIENCE, 1984, 226 (4674) : 544 - 547
  • [9] Cellular and immunological basis of the host-parasite relationship during infection with Neospora caninum
    Hemphill, A.
    Vonlaufen, N.
    Naguleswaran, A.
    [J]. PARASITOLOGY, 2006, 133 : 261 - 278
  • [10] HIGH KP, 1994, J BIOL CHEM, V269, P9105
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