A dynamic microbial community with high functional redundancy inhabits the cold, oxic subseafloor aquifer
被引:107
作者:
Tully, Benjamin J.
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机构:
Univ Southern Calif, Ctr Dark Energy Biosphere Invest, 3616 Trousdale Pkwy, Los Angeles, CA 90089 USAUniv Southern Calif, Ctr Dark Energy Biosphere Invest, 3616 Trousdale Pkwy, Los Angeles, CA 90089 USA
Tully, Benjamin J.
[1
]
Wheat, C. Geoff
论文数: 0引用数: 0
h-index: 0
机构:
Univ Alaska Fairbanks, Coll Fisheries & Ocean Sci, Fairbanks, AK 99701 USAUniv Southern Calif, Ctr Dark Energy Biosphere Invest, 3616 Trousdale Pkwy, Los Angeles, CA 90089 USA
Wheat, C. Geoff
[2
]
Glazer, Brain T.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hawaii, Dept Oceanog, Honolulu, HI 96822 USAUniv Southern Calif, Ctr Dark Energy Biosphere Invest, 3616 Trousdale Pkwy, Los Angeles, CA 90089 USA
Glazer, Brain T.
[3
]
Huber, Julie A.
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h-index: 0
机构:
Josephine Bay Paul Ctr, Marine Biol Lab, Woods Hole, MA USA
Woods Hole Oceanog Inst, Marine Chem & Geochem, Woods Hole, MA 02543 USAUniv Southern Calif, Ctr Dark Energy Biosphere Invest, 3616 Trousdale Pkwy, Los Angeles, CA 90089 USA
Huber, Julie A.
[4
,5
]
机构:
[1] Univ Southern Calif, Ctr Dark Energy Biosphere Invest, 3616 Trousdale Pkwy, Los Angeles, CA 90089 USA
[2] Univ Alaska Fairbanks, Coll Fisheries & Ocean Sci, Fairbanks, AK 99701 USA
[3] Univ Hawaii, Dept Oceanog, Honolulu, HI 96822 USA
[4] Josephine Bay Paul Ctr, Marine Biol Lab, Woods Hole, MA USA
[5] Woods Hole Oceanog Inst, Marine Chem & Geochem, Woods Hole, MA 02543 USA
The rock-hosted subseafloor crustal aquifer harbors a reservoir of microbial life that may influence global marine biogeochemical cycles. Here we utilized metagenomic libraries of crustal fluid samples from North Pond, located on the flanks of the Mid-Atlantic Ridge, a site with cold, oxic subseafloor fluid circulation within the upper basement to query microbial diversity. Twenty-one samples were collected during a 2-year period to examine potential microbial metabolism and community dynamics. We observed minor changes in the geochemical signatures over the 2 years, yet the microbial community present in the crustal fluids underwent large shifts in the dominant taxonomic groups. An analysis of 195 metagenome-assembled genomes (MAGs) were generated from the data set and revealed a connection between litho-and autotrophic processes, linking carbon fixation to the oxidation of sulfide, sulfur, thiosulfate, hydrogen, and ferrous iron in members of the Proteobacteria, specifically the Alpha-, Gamma-and Zetaproteobacteria, the Epsilonbacteraeota and the Planctomycetes. Despite oxic conditions, analysis of the MAGs indicated that members of the microbial community were poised to exploit hypoxic or anoxic conditions through the use of microaerobic cytochromes, such as cbb(3)- and bd-type cytochromes, and alternative electron acceptors, like nitrate and sulfate. Temporal and spatial trends from the MAGs revealed a high degree of functional redundancy that did not correlate with the shifting microbial community membership, suggesting functional stability in mediating subseafloor biogeochemical cycles. Collectively, the repeated sampling at multiple sites, together with the successful binning of hundreds of genomes, provides an unprecedented data set for investigation of microbial communities in the cold, oxic crustal aquifer.
机构:
Univ So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USAUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
Robador, Alberto
LaRowe, Douglas E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ So Calif, Dept Earth Sci, Los Angeles, CA USAUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
LaRowe, Douglas E.
Jungbluth, Sean P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hawaii Manoa, Dept Oceanog, Sch Ocean & Earth Sci & Technol, Honolulu, HI 96822 USA
Univ Hawaii Manoa, Hawaii Inst Marne Biol, Sch Ocean & Earth Sci & Technol, Kaneohe, HI USA
Univ So Calif, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USAUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
Jungbluth, Sean P.
Lin, Huei-Ting
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hawaii Manoa, Dept Oceanog, Sch Ocean & Earth Sci & Technol, Honolulu, HI 96822 USA
Natl Taiwan Univ, Inst Oceanog, Taipei 10764, TaiwanUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
Lin, Huei-Ting
Rappe, Michael S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hawaii Manoa, Hawaii Inst Marne Biol, Sch Ocean & Earth Sci & Technol, Kaneohe, HI USAUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
Rappe, Michael S.
Nealson, Kenneth H.
论文数: 0引用数: 0
h-index: 0
机构:
Univ So Calif, Dept Earth Sci, Los Angeles, CA USA
Univ So Calif, Dept Biol Sci, Los Angeles, CA 90089 USAUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
Nealson, Kenneth H.
Amend, Jan P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ So Calif, Dept Earth Sci, Los Angeles, CA USA
Univ So Calif, Dept Biol Sci, Los Angeles, CA 90089 USAUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
机构:
Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Ohio State Univ, Plant Mol Biol Biotechnol Program, Columbus, OH 43210 USAOhio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Tabita, F. Robert
Hanson, Thomas E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Delaware, Delaware Biotechnol Inst, Grad Coll Marine & Earth Studies, Newark, DE 19711 USAOhio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Hanson, Thomas E.
Li, Huiying
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Los Angeles, Dept Chem & Biochem, UCLA DOE Inst Genom & Proteom, Howard Hughes Med Inst, Los Angeles, CA 90095 USAOhio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Li, Huiying
Satagopan, Srirarn
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Ohio State Univ, Plant Mol Biol Biotechnol Program, Columbus, OH 43210 USAOhio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Satagopan, Srirarn
Singh, Jaya
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Aronoff Lab 582, Dept Plant Cellular & Mol Biol, Columbus, OH 43210 USAOhio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Singh, Jaya
Chan, Sum
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Los Angeles, Dept Chem & Biochem, UCLA DOE Inst Genom & Proteom, Howard Hughes Med Inst, Los Angeles, CA 90095 USAOhio State Univ, Dept Microbiol, Columbus, OH 43210 USA
机构:
Univ Southern Calif, Ctr Dark Energy Biosphere Invest, Los Angeles, CA 90089 USAUniv Southern Calif, Ctr Dark Energy Biosphere Invest, Los Angeles, CA 90089 USA
Tully, Benjamin J.
Heidelberg, John F.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Southern Calif, Ctr Dark Energy Biosphere Invest, Los Angeles, CA 90089 USA
Univ Southern Calif, Dept Biol Sci, Los Angeles, CA USAUniv Southern Calif, Ctr Dark Energy Biosphere Invest, Los Angeles, CA 90089 USA
机构:
Univ Alaska Fairbanks, POB 475, Moss Landing, CA 95039 USAUniv Alaska Fairbanks, POB 475, Moss Landing, CA 95039 USA
Wheat, C. Geoffrey
Fisher, Andrew T.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Santa Cruz, Earth & Planetary Sci Dept, 1156 High St, Santa Cruz, CA 95064 USAUniv Alaska Fairbanks, POB 475, Moss Landing, CA 95039 USA
Fisher, Andrew T.
McManus, James
论文数: 0引用数: 0
h-index: 0
机构:
Univ Akron, Dept Geosci, Akron, OH 44326 USA
Bigelow Lab Ocean Sci, 60 Bigelow Dr, East Boothbay, ME 04544 USAUniv Alaska Fairbanks, POB 475, Moss Landing, CA 95039 USA
McManus, James
Hulme, Samuel M.
论文数: 0引用数: 0
h-index: 0
机构:
Moss Landing Marine Lab, Moss Landing, CA 95039 USAUniv Alaska Fairbanks, POB 475, Moss Landing, CA 95039 USA
Hulme, Samuel M.
Orcutt, Beth N.
论文数: 0引用数: 0
h-index: 0
机构:
Bigelow Lab Ocean Sci, 60 Bigelow Dr, East Boothbay, ME 04544 USAUniv Alaska Fairbanks, POB 475, Moss Landing, CA 95039 USA
机构:
Univ So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USAUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
Robador, Alberto
LaRowe, Douglas E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ So Calif, Dept Earth Sci, Los Angeles, CA USAUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
LaRowe, Douglas E.
Jungbluth, Sean P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hawaii Manoa, Dept Oceanog, Sch Ocean & Earth Sci & Technol, Honolulu, HI 96822 USA
Univ Hawaii Manoa, Hawaii Inst Marne Biol, Sch Ocean & Earth Sci & Technol, Kaneohe, HI USA
Univ So Calif, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USAUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
Jungbluth, Sean P.
Lin, Huei-Ting
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hawaii Manoa, Dept Oceanog, Sch Ocean & Earth Sci & Technol, Honolulu, HI 96822 USA
Natl Taiwan Univ, Inst Oceanog, Taipei 10764, TaiwanUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
Lin, Huei-Ting
Rappe, Michael S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hawaii Manoa, Hawaii Inst Marne Biol, Sch Ocean & Earth Sci & Technol, Kaneohe, HI USAUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
Rappe, Michael S.
Nealson, Kenneth H.
论文数: 0引用数: 0
h-index: 0
机构:
Univ So Calif, Dept Earth Sci, Los Angeles, CA USA
Univ So Calif, Dept Biol Sci, Los Angeles, CA 90089 USAUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
Nealson, Kenneth H.
Amend, Jan P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ So Calif, Dept Earth Sci, Los Angeles, CA USA
Univ So Calif, Dept Biol Sci, Los Angeles, CA 90089 USAUniv So Calif, NASA Astrobiol Inst, Ctr Dark Energy Biosphere Invest, Los Angeles, CA USA
机构:
Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Ohio State Univ, Plant Mol Biol Biotechnol Program, Columbus, OH 43210 USAOhio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Tabita, F. Robert
Hanson, Thomas E.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Delaware, Delaware Biotechnol Inst, Grad Coll Marine & Earth Studies, Newark, DE 19711 USAOhio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Hanson, Thomas E.
Li, Huiying
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Los Angeles, Dept Chem & Biochem, UCLA DOE Inst Genom & Proteom, Howard Hughes Med Inst, Los Angeles, CA 90095 USAOhio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Li, Huiying
Satagopan, Srirarn
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Ohio State Univ, Plant Mol Biol Biotechnol Program, Columbus, OH 43210 USAOhio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Satagopan, Srirarn
Singh, Jaya
论文数: 0引用数: 0
h-index: 0
机构:
Ohio State Univ, Aronoff Lab 582, Dept Plant Cellular & Mol Biol, Columbus, OH 43210 USAOhio State Univ, Dept Microbiol, Columbus, OH 43210 USA
Singh, Jaya
Chan, Sum
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Los Angeles, Dept Chem & Biochem, UCLA DOE Inst Genom & Proteom, Howard Hughes Med Inst, Los Angeles, CA 90095 USAOhio State Univ, Dept Microbiol, Columbus, OH 43210 USA
机构:
Univ Southern Calif, Ctr Dark Energy Biosphere Invest, Los Angeles, CA 90089 USAUniv Southern Calif, Ctr Dark Energy Biosphere Invest, Los Angeles, CA 90089 USA
Tully, Benjamin J.
Heidelberg, John F.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Southern Calif, Ctr Dark Energy Biosphere Invest, Los Angeles, CA 90089 USA
Univ Southern Calif, Dept Biol Sci, Los Angeles, CA USAUniv Southern Calif, Ctr Dark Energy Biosphere Invest, Los Angeles, CA 90089 USA
机构:
Univ Alaska Fairbanks, POB 475, Moss Landing, CA 95039 USAUniv Alaska Fairbanks, POB 475, Moss Landing, CA 95039 USA
Wheat, C. Geoffrey
Fisher, Andrew T.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Santa Cruz, Earth & Planetary Sci Dept, 1156 High St, Santa Cruz, CA 95064 USAUniv Alaska Fairbanks, POB 475, Moss Landing, CA 95039 USA
Fisher, Andrew T.
McManus, James
论文数: 0引用数: 0
h-index: 0
机构:
Univ Akron, Dept Geosci, Akron, OH 44326 USA
Bigelow Lab Ocean Sci, 60 Bigelow Dr, East Boothbay, ME 04544 USAUniv Alaska Fairbanks, POB 475, Moss Landing, CA 95039 USA
McManus, James
Hulme, Samuel M.
论文数: 0引用数: 0
h-index: 0
机构:
Moss Landing Marine Lab, Moss Landing, CA 95039 USAUniv Alaska Fairbanks, POB 475, Moss Landing, CA 95039 USA
Hulme, Samuel M.
Orcutt, Beth N.
论文数: 0引用数: 0
h-index: 0
机构:
Bigelow Lab Ocean Sci, 60 Bigelow Dr, East Boothbay, ME 04544 USAUniv Alaska Fairbanks, POB 475, Moss Landing, CA 95039 USA