A Portable Multi-Channel Turbidity System for Rapid Detection of Pathogens by Loop-Mediated Isothermal Amplification

被引：104
作者：
Chen, Zhu [1 ,2 ]
Yang, Tong [2 ]
Yang, Haowen [2 ,3 ]
Li, Taotao [4 ]
Nie, Libo [1 ]
Mou, Xianbo [5 ]
Deng, Yan [1 ]
He, Nongyue [1 ,2 ]
Li, Zhiyang [6 ]
Wang, Lijun [7 ,8 ,9 ]
Li, Song [1 ]
机构：
[1] Hunan Univ Technol, Hunan Key Lab Biomed Nanomat & Devices, Econ Forest Cultivat & Utilizat Collaborat Innova, Zhuzhou 412007, Peoples R China
[2] Southeast Univ, Natl Demonstrat Ctr Expt Biomed Engn Educ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
[3] Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, CH-4058 Basel, Switzerland
[4] Hunan City Univ, Sch Mat & Chem Engn, Hunan Prov Key Lab Dark Tea & Jin Hua, Yiyang 413000, Peoples R China
[5] Ningbo Univ, Med Sch, Ningbo 315211, Zhejiang, Peoples R China
[6] Nanjing Univ, Med Sch, Affiliated Drum Tower Hosp, Dept Clin Lab, Nanjing 210008, Jiangsu, Peoples R China
[7] Guangxi Univ, Coll Light Ind & Food Engn, Nanning 530004, Peoples R China
[8] Guangxi Key Lab Clean Pulp & Paper Making & Pollu, Nanning 530007, Peoples R China
[9] Collaborat Innovat Ctr Guangxi Sugar Ind, Nanning 530004, Peoples R China
来源：
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | 2018年 / 14卷 / 01期
基金：
中国博士后科学基金;
关键词：
Loop-Mediated Isothermal Amplification; Turbidimeter; Legionella; H7N9; Optical Detector; POLYMERASE-CHAIN-REACTION; MAGNETIC NANOPARTICLES; MICROFLUIDIC CHIP; SAMPLE; MICROARRAY; BIOSENSOR; DESIGN; CANCER; DEVICE; ANSWER;
D O I：
10.1166/jbn.2018.2524
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
This study aimed at developing a portable multi-channel turbidity system (21 cm in length, 15.5 cm in width and 11.5 cm in depth) by real-time loop-mediated isothermal amplification (LAMP) method for rapid detection of pathogens. The developed system herein includes temperature control unit, photoelectric detection unit, turbidity calibration unit, power management unit, human machine unit, communication unit and ARM-based microcontroller. The coefficient of variation for eight channels is less than 0.25% in noise analysis. Legionella bacteria (LEG) and H7 subtype virus (H7) were successively detected by the designed and developed system within 60 minutes. Moreover, its specificity for LEG is satisfactory and its sensitivity for H7 is 10 copies/mL. Besides, this system for point-of-care diagnosis allows a rapid, small size, low cost, and automatic detection with the characteristics of high-efficiency, excellent stability and high uniformity.
引用
收藏
页码：198 / 205
页数：8
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