Polymorphisms in the S1 spike glycoprotein of Arkansas-type infectious bronchitis virus (IBV) show differential binding to host tissues and altered antigenicity

被引：21
作者：
Leyson, Christina [1 ]
Franca, Monique [1 ]
Jackwood, Mark [1 ]
Jordan, Brian [1 ,2 ]
机构：
[1] Univ Georgia, Dept Populat Hlth, Coll Vet Med, Poultry Diagnost & Res Ctr, 953 Coll Stn Rd, Athens, GA 30602 USA
[2] Univ Georgia, Dept Poultry Sci, Coll Agr & Environm Sci, 953 Coll Stn Rd, Athens, GA 30602 USA
来源：
VIROLOGY | 2016年 / 498卷
关键词：
Infectious bronchitis virus; Arkansas-type vaccine; Spike glycoprotein; S1; spike; Coronavirus spike; CORONAVIRUS IBV; MONOCLONAL-ANTIBODIES; PEPLOMER PROTEIN; AMINO-ACIDS; SUBPOPULATIONS; SELECTION; CHICKENS; STRAINS; ATTACHMENT; EVOLUTION;
D O I：
10.1016/j.virol.2016.08.030
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Sequencing avian infectious bronchitis virus spike genes re-isolated from vaccinated chicks revealed that many sequence changes are found on the S1 spike gene. In the ArkDPI strain, Y43H and Delta 344 are the two most common changes observed. This study aims to examine the roles of Y43H and Delta 344 in selection in vivo. Using recombinant ArkDPI S1 proteins, we conducted binding assays on chicken tracheas and embryonic chorioallantoic membrane (CAM). Protein histochemistry showed that the Y43H change allows for enhanced binding to trachea, whereas the ArkDPI S1 spike with H43 alone was able to bind CAM. Using Western blot under denaturing conditions, ArkDPI serotype-specific sera did not bind to S1 proteins with Delta 344, suggesting that Delta 344 alters antigenicity of S1. These findings are important because they propose that specific changes in S1 enhances virus fitness by more effective binding to host tissues (Y43H) and by evading a vaccine-induced antibody response (Delta 344). (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码：218 / 225
页数：8
相关论文
共 37 条
[1]   Identification of sequence changes responsible for the attenuation of avian infectious bronchitis virus strain Arkansas DPI [J].
Ammayappan, Arun ;
Upadhyay, Chitra ;
Gelb, Jack, Jr. ;
Vakharia, Vikram N. .
ARCHIVES OF VIROLOGY, 2009, 154 (03) :495-499
[2]  
Becker W B, 1967, J Virol, V1, P1019
[3]   The coronavirus spike protein is a class I virus fusion protein: Structural and functional characterization of the fusion core complex [J].
Bosch, BJ ;
van der Zee, R ;
de Haan, CAM ;
Rottier, PJM .
JOURNAL OF VIROLOGY, 2003, 77 (16) :8801-8811
[4]   Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism [J].
Casais, R ;
Dove, B ;
Cavanagh, D ;
Britton, P .
JOURNAL OF VIROLOGY, 2003, 77 (16) :9084-9089
[5]   CORONAVIRUS IBV - REMOVAL OF SPIKE GLYCOPOLYPEPTIDE-S1 BY UREA ABOLISHES INFECTIVITY AND HEMAGGLUTINATION BUT NOT ATTACHMENT TO CELLS [J].
CAVANAGH, D ;
DAVIS, PJ .
JOURNAL OF GENERAL VIROLOGY, 1986, 67 :1443-1448
[6]   AMINO-ACIDS WITHIN HYPERVARIABLE REGION-1 OF AVIAN CORONAVIRUS IBV (MASSACHUSETTS SEROTYPE) SPIKE GLYCOPROTEIN ARE ASSOCIATED WITH NEUTRALIZATION EPITOPES [J].
CAVANAGH, D ;
DAVIS, PJ ;
MOCKETT, APA .
VIRUS RESEARCH, 1988, 11 (02) :141-150
[7]   Coronavirus avian infectious bronchitis virus [J].
Cavanagh, Dave .
VETERINARY RESEARCH, 2007, 38 (02) :281-297
[8]   A Single Immunization with Soluble Recombinant Trimeric Hemagglutinin Protects Chickens against Highly Pathogenic Avian Influenza Virus H5N1 [J].
Cornelissen, Lisette A. H. M. ;
de Vries, Robert P. ;
de Boer-Luijtze, Els A. ;
Rigter, Alan ;
Rottier, Peter J. M. ;
de Haan, Cornelis A. M. .
PLOS ONE, 2010, 5 (05)
[9]   ASSESSMENT OF CROSS-IMMUNITY IN CHICKENS TO STRAINS OF AVIAN INFECTIOUS-BRONCHITIS VIRUS USING TRACHEAL ORGAN-CULTURES [J].
DARBYSHIRE, JH .
AVIAN PATHOLOGY, 1980, 9 (02) :179-184
[10]   Infectious bronchitis virus variants: a review of the history, current situation and control measures [J].
de Wit, J. J. ;
Cook, Jane K. A. ;
van der Heijden, Harold M. J. F. .
AVIAN PATHOLOGY, 2011, 40 (03) :223-235
1234