Optimization of ultrasound-assisted extraction of naturally occurring glucosinolates from by-products of Camelina sativa L. and their effect on human colorectal cancer cell line

被引：13

作者：

Pagliari, Stefania ^{[1
]}

Giustra, Chiara Maria ^{[1
]}

Magoni, Chiara ^{[1
]}

Celano, Rita ^{[2
]}

Fusi, Paola ^{[1
]}

Forcella, Matilde ^{[1
]}

Sacco, Grazia ^{[1
]}

Panzeri, Davide ^{[1
]}

Campone, Luca ^{[1
]}

Labra, Massimo ^{[1
]}

机构：

[1] Univ Milano Bicocca, Dept Biotechnol & Biosci, Milan, Italy

[2] Univ Salerno, Dept Pharm, Fisciano, Italy

来源：

FRONTIERS IN NUTRITION | 2022年 / 9卷

关键词：

Camelina sativa L; ultrasound-assisted extraction (USAE); experimental design optimization; human colorectal cancer cell line; recovery bioactive compounds; glucosinolates derivatives; food by-products; THERMAL-DEGRADATION; ISOTHIOCYANATES; VEGETABLES; DIVERSITY; PROFILES; TISSUES; RISK;

D O I：

10.3389/fnut.2022.901944

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

The food waste generated by small and medium agro-industrial enterprises requires appropriate management and valorization in order to decrease environmental problems and recover high-value products, respectively. In this study, the Camelina sativa seed by-product was used as a source of glucosinolates. To begin, the chemical profile of the extract obtained using an international organization for standardization (ISO) procedure was determined by UPLC-HRMS/MS analysis. In addition, an extraction method based on ultrasound-assisted extraction was developed as an alternative and green method to recover glucosinolates. Main parameters that affect extraction efficiency were optimized using a response surface design. Under optimized conditions, the extract showed an improvement in extraction yield with a reduction in organic solvent amount compared to those obtained using the ISO procedure. Finally, the extract obtained with the ultrasound-assisted method was purified, tested on human colorectal cancer cell lines, and showed promising results.

引用

页数：11

共 34 条

[1] Optimized analysis and quantification of glucosinolates from Camelina sativa seeds by reverse-phase liquid chromatography [J].