An optimal method of DNA silver staining in polyacrylamide gels

被引：33

作者：

Han, Yan-Chuang ^{[1
]}

Teng, Cai-Zhu ^{[2
]}

Hu, Zhong-Li ^{[1
]}

Song, Yun-Chun ^{[1
]}

机构：

[1] Wuhan Univ, Key Lab Minist Educ Plant Dev, Coll Life Sci, Wuhan 430072, Peoples R China

[2] Guangxi Normal Univ, Coll Life Sci, Guilin, Peoples R China

来源：

ELECTROPHORESIS | 2008年 / 29卷 / 06期

关键词：

DNA; Eriochrome black T; polyacrylamide gel; silver staining;

D O I：

10.1002/elps.200700558

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA silver staining has widely been used to detect DNA fragments in polyacrylamide gels with high sensitivity. We developed an optimal method for DNA silver staining on polyacrylamide gels. The novel procedure can be completed within 10 min instead of over 20 min with the conventional methods. The sensitivity is significantly improved by the silver-ion sensitizer (Eriochrome black T (EBT)) and the minimum of 0.11 and 1.75 ng of DNA amount can be detected in denaturing and nondenaturing polyacrylamide gel, respectively. Compared with the conventional silver staining methods, the improved optimal method can save time and display high sensitivity, color uniformity, and long storage time of the staining gels.

引用

页码：1355 / 1358

页数：4

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