Characterizing protein modifications by reactive metabolites using magnetic bead bioreactors and LC-MS/MS

被引：3

作者：

Li, Dandan ^{[1
]}

Fu, You-Jun ^{[1
]}

Rusling, James F. ^{[1
,2
,3
]}

机构：

[1] Univ Connecticut, Dept Chem, Storrs, CT 06269 USA

[2] Natl Univ Ireland Galway, Galway, Ireland

[3] Univ Connecticut, Ctr Hlth, Dept Cell Biol, Farmington, CT 06032 USA

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 22期

关键词：

GLUTATHIONE-S-TRANSFERASE; INDUCED HEPATIC-NECROSIS; MASS-SPECTROMETRY; COVALENT BINDING; TOXICITY; ACETAMINOPHEN; MECHANISMS; DRUG; ELECTROPHILES; PARACETAMOL;

D O I：

10.1039/c5cc00420a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report here label-free metabolite-protein adduct detection and identification employing magnetic beads coated with metabolic enzymes as bioreactors to generate metabolites and possible metabolite-protein adducts for analysis by liquid chromatographytandem mass spectrometry.

引用

收藏

页码：4701 / 4703

页数：3

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