Characterization of κ-opioid receptor transcripts expressed by T cells and macrophages

被引：38

作者：

Alicea, C

Belkowski, SM

Sliker, JK

Zhu, JM

Liu-Chen, LY

Eisenstein, TK

Adler, MW

Rogers, TJ

机构：

[1] Temple Univ, Sch Med, Dept Microbiol & Immunol, Philadelphia, PA 19140 USA

[2] Temple Univ, Sch Med, Dept Pharmacol, Philadelphia, PA 19140 USA

[3] Temple Univ, Sch Med, Fels Inst Canc Res & Mol Biol, Philadelphia, PA 19140 USA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1998年 / 91卷 / 1-2期

关键词：

kappa-opioid receptor; R1.1; thymoma; DPK cell line; macrophages; RT-PCR; truncated transcript;

D O I：

10.1016/S0165-5728(98)00151-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have found that the immature T cell lines R1.1 and DPK and the macrophage lines P388D(1) and WEHI-S also express K-opioid receptor (KOR) mRNA. Characterization of the KOR transcripts in both brain tissue and these T cells has revealed both the normal full-length as well as a truncated form of the mRNA. Our results show that the truncated transcript lacks the second exon. Primary macrophages express this truncated form of the transcript in the absence of detectable levels of the full-length form. These results suggest a degree of heterogeneity in the expression of the opioid receptors which has not previously been reported. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：55 / 62

页数：8

共 38 条

[1] Inhibition of primary murine macrophage cytokine production in vitro following treatment with the kappa-opioid agonist U50,488H [J].