High-performance Liquid Chromatographic Determination and Pharmacokinetic Study of Apigenin-7-O-β-D-glucoside in Rat Plasma after Intravenous Administration

被引：12

作者：

Chen, Zaixing ^{[1
]}

Ying, Xixiang ^{[2
]}

Meng, Shu ^{[3
]}

Zhu, Xu ^{[1
]}

Jiang, Hong ^{[4
]}

Cao, Qishen ^{[2
]}

Li, Xuying ^{[1
]}

Meng, Fanhao ^{[1
]}

机构：

[1] China Med Univ, Sch Pharmaceut Sci, Shenyang 110001, Liaoning Prov, Peoples R China

[2] Liaoning Univ Tradit Chinese Med, Sch Pharm, Dalian 110032, Liaoning Prov, Peoples R China

[3] Liaoning Inst Family Planning, Shenyang 110031, Peoples R China

[4] China Med Univ, Sch Publ Hlth, Shenyang 110001, Peoples R China

来源：

ARCHIVES OF PHARMACAL RESEARCH | 2011年 / 34卷 / 05期

关键词：

Apigenin-7-O-beta-D-glucoside; Pharmacokinetics; High performance liquid chromatography; POLYPHENOLS; LUTEOLIN;

D O I：

10.1007/s12272-011-0507-3

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The present study was designed to validate an analytical method based on HPLC for the quantitative measurement of the Apigenin-7-O-beta-D-glucoside (AGL) in rat plasma after intravenous administration. HPLC analysis was done using a C-18 column, UV detection at 335 nm, and a mobile phase of methanol:0.2% phosphoric acid (1:1, v/v). Good linearity was observed over the range of 0.06-7.20 mu g/mL with a lower limit of quantication of 0.06 mu g/mL. The intra- and inter-day precision values were below 9.97%. No interference peaks or matrix effects were observed. The method was fully validated and successfully applied to a pharmacokinetic study of AGL.

引用

页码：741 / 746

页数：6

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