共 11 条
[11]
Yamamoto S., 1994, Oyo Toshitsu Kagaku = Journal of Applied Glycoscience, V41, P283
Simple affinity purification method for raw starch-adsorbable and -digesting amylases with a raw starch column
被引：0
作者：
Mai, HT
Furuyoshi, S
Yamamoto, S
Yagi, T
机构：
[1] KOCHI UNIV, FAC AGR, DEPT BIORESOURCE SCI, NANKOKU, KOCHI 783, JAPAN
[2] KOCHI UNIV, RES INST MOL GENET, NANKOKU, KOCHI 783, JAPAN
关键词：
amylase;
raw starch;
affinity chromatography;
raw starch-digesting amylase;
D O I：
暂无
中图分类号：
Q5 [生物化学];
Q7 [分子生物学];
学科分类号：
071010 ;
081704 ;
摘要：
A simple purification procedure for raw starch-adsorbable and -digesting amylases (RSAs) was devised. The method depended on an affinity column, which was prepared by mixing raw corn starch and Hyflo Super-Gel. RSAs were specifically adsorbed on the matrix, and eluted with a buffer containing 1% beta-cyclodextrin. This column could be used to purify RSAs from Streptomyces thermocyaneoviolaceus and a recombinant strain of E. coli.
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页码：1717 / 1719
页数：3
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