Simple affinity purification method for raw starch-adsorbable and -digesting amylases with a raw starch column

被引：0

作者：

Mai, HT

Furuyoshi, S

Yamamoto, S

Yagi, T

机构：

[1] KOCHI UNIV, FAC AGR, DEPT BIORESOURCE SCI, NANKOKU, KOCHI 783, JAPAN

[2] KOCHI UNIV, RES INST MOL GENET, NANKOKU, KOCHI 783, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 10期

关键词：

amylase; raw starch; affinity chromatography; raw starch-digesting amylase;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple purification procedure for raw starch-adsorbable and -digesting amylases (RSAs) was devised. The method depended on an affinity column, which was prepared by mixing raw corn starch and Hyflo Super-Gel. RSAs were specifically adsorbed on the matrix, and eluted with a buffer containing 1% beta-cyclodextrin. This column could be used to purify RSAs from Streptomyces thermocyaneoviolaceus and a recombinant strain of E. coli.

引用

页码：1717 / 1719

页数：3

共 11 条

[1]

Dubois M., 1956, ANAL CHEM, V28, P305

[2]

Fogarty W. M., 1990, MICROBIAL ENZYMES BI, P71

[3] SPECIFIC-INHIBITION BY CYCLODEXTRINS OF RAW STARCH DIGESTION BY FUNGAL GLUCOAMYLASE [J].