Cytotoxicity of contact lens multipurpose solutions: Role of oxidative stress, mitochondrial activity and P2X7 cell death receptor activation

Objective: To investigate the cytotoxicity of multipurpose solutions used for contact lens disinfection. Methods: Four multipurpose solutions (Optifree (R) containing quaternary ammonium as preservative, Renu (R), Solocare (R) and Complete (R) containing polyhexamethylene biguanide as preservative) were incubated on a conjunctival cell line to evaluate their capacity to induce necrosis (neutral red test), intracellular redox status alteration (Alamar Blue test), reactive oxygen species overproduction (DCFH-DA and dihydroethidium tests), mitochondrial alterations (NonylAcridine Orange and JC-1 tests) and to activate P2X7 cell death receptor (YO-PRO-1 test). Tests were performed using cytofluorometry and inverted fluorescence microscopy. Results: Our results showed that multipurpose solutions induced necrosis, in addition to oxidative stress. Optifree (R) induced oxidative stress with increased mitochondrial mass, Renu (R), Solocare (R) and Complete (R) induced whether a decrease in reactive oxygen species production with mitochondrial alterations, or an increase in reactive oxygen species production, but each solution stimulated P2X7 cell death receptor activation. Early stimulation of P2X7 cell death receptor, prior to any superoxide production, demonstrated the possible involvement of this receptor in the oxidative stress process. Conclusion: These new findings suggest that most of multipurpose solutions are toxic with oxidative stress and apoptosis induction. (c) 2007 Elsevier B.V. All rights reserved.