Alamandine protects against renal ischaemia-reperfusion injury in rats via inhibiting oxidative stress

被引:14
作者
Zhu, Jue [1 ]
Qiu, Jian-Guo [2 ]
Xu, Wei-Tao [3 ]
Ma, Hong-Xiang [4 ]
Jiang, Ke [4 ]
机构
[1] Peoples Hosp Liyang, Dept Nephrol, Changzhou, Peoples R China
[2] Nanjing Med Univ, Kangda Coll, Lianshui Peoples Hosp, Dept Urol, Huaian, Peoples R China
[3] Zaozhuang Min Grp Cent Hosp, Dept Nephrol, Zaozhuang, Peoples R China
[4] Peoples Hosp Liyang, Dept Urol, 70 Jianshe West Rd, Changzhou 213399, Jiangsu, Peoples R China
关键词
alamandine; renal ischaemia and reperfusion; inflammation; apoptosis; oxidative stress; NADPH oxidase 1; ISCHEMIA/REPERFUSION INJURY; APOPTOSIS; ANGIOTENSIN-(1-7); INFLAMMATION; AUTOPHAGY; FAILURE; MICE;
D O I
10.1093/jpp/rgab091
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Objective This study was to determine whether alamandine (Ala) could reduce ischaemia and reperfusion (I/R) injury of kidney in rats. Methods Renal I/R was induced by an occlusion of bilateral renal arteries for 70 min and a 24-h reperfusion in vivo, and rat kidney proximal tubular epithelial cells NRK52E were exposed to 24 h of hypoxia and followed by 3-h reoxygenation (H/R) in vitro. Results The elevated serum creatinine (Cr), blood cystatin C (CysC) and blood urea nitrogen (BUN) levels in I/R rats were inhibited by Ala treatment.Tumour necrosis factor alpha (TNF)-alpha, IL-1 beta, IL-6, cleaved caspase-3, cleaved caspase-8 and Bax were increased, and BcI2 was reduced in the kidney of I/R rats, which were reversed by Ala administration. Ala reversed the increase of TNF-alpha, IL-beta, cleaved caspase-3, cleaved caspase-8 and Bax and the decrease of Bcl2 in the H/R NRK52E cells. Ala could also inhibit the increase of oxidative stress levels in the kidney of I/R rats. NADPH oxidase 1 (Nox1) overexpression reversed the improving effects of Ala on renal function, inflammation and apoptosis of I/R rats. Conclusion These results indicated that Ala could improve renal function, attenuate inflammation and apoptosis in the kidney of I/R rats via inhibiting oxidative stress.
引用
收藏
页码:1491 / 1502
页数:12
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