Experimental visualization of mixing pathologies in laminar stirred tank bioreactors

被引：37

作者：

Alvarez, MM

Guzmán, A

Elías, M

机构：

[1] Inst Tecnol & Estudios Superiores Monterrey, Ctr Biotecnol, Monterrey 64849, Mexico

[2] Univ Autonoma Nuevo Leon, Planta Piloto Fermentaciones, Monterrey, NL, Mexico

来源：

CHEMICAL ENGINEERING SCIENCE | 2005年 / 60卷 / 8-9期

关键词：

D O I：

10.1016/j.ces.2004.11.049

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Most of the bio-reactive processes now in development by Pharmaceutical and Biotechnological firms will be based on the culture of shear-sensitive mammalian. insect or plant cells. To properly address the need of designing, optimizing, and scaling up such bioprocesses, a precise characterization of the flow and mixing behavior of typical and novel bioreactor geometries is required. Using experimental visualization techniques (3-D UV light pattern visualization, 2-D laser-induced fluorescence techniques, and acid base reactions in the presence of pH indicators) we experimentally describe and analyze common mixing pathologies occurring in frequently used bioreactor geometries: heterogeneity, coexistence of regular and chaotic regions, flow segregation, compartmentalization, flow bypass, cell focusing, etc. We also analyze the role of baffles on mixing in bioreactors operated at low and moderate Re. and discuss the risk and validity of assuming perfectly mixed conditions in a bio-reaction system. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：2449 / 2457

页数：9

共 29 条

[1]

ALOIA JF, 1994, ADV NUTR RES, V9, P1

[2] Self-similar spatiotemporal structure of intermaterial boundaries in chaotic flows [J].

Alvarez, MM ;

Muzzio, FJ ;

Cerbelli, S ;

Adrover, A ;

Giona, M .

PHYSICAL REVIEW LETTERS, 1998, 81 (16) :3395-3398

[3] Mechanisms of mixing and creation of structure in laminar stirred tanks [J].

Alvarez, MM ;

Zalc, JM ;

Shinbrot, T ;

Arratia, PE ;

Muzzio, FJ .

AICHE JOURNAL, 2002, 48 (10) :2135-2148

[4]

ALVAREZ MM, 2000, THESIS STATE U NEW J

[5] Practical chaotic mixing [J].

Alvarez-Hernández, MM ;

Shinbrot, T ;

Zalc, J ;

Muzzio, FJ .

CHEMICAL ENGINEERING SCIENCE, 2002, 57 (17) :3749-3753

[6] Effect of mixing on enzymatic liquefaction of sage starch [J].

Ariff, AB ;

Asbi, BA ;

Azudin, MN ;

Kennedy, JF .

CARBOHYDRATE POLYMERS, 1997, 33 (2-3) :101-108

[7] Segregated regions in continuous laminar stirred tank reactors [J].

Arratia, PE ;

Lacombe, JP ;

Shinbrot, T ;

Muzzio, FJ .

CHEMICAL ENGINEERING SCIENCE, 2004, 59 (07) :1481-1490

[8] Enhanced mixing and mass transfer in a recirculation loop results in high cell densities in a roller bottle reactor [J].

Berson, RE ;

Pieczynski, WJ ;

Svihla, CK ;

Hanley, TR .

BIOTECHNOLOGY PROGRESS, 2002, 18 (01) :72-77

[9] TURBULENT SHEAR-STRESS EFFECT ON MAMMALIAN-CELL CULTURE AND MEASUREMENT USING LASER-DOPPLER ANEMOMETER [J].

ELIAS, CB ;

DESAI, RB ;

PATOLE, MS ;

JOSHI, JB ;

MASHELKAR, RA .

CHEMICAL ENGINEERING SCIENCE, 1995, 50 (15) :2431-+

[10] Physiological responses to mixing in large scale bioreactors [J].

Enfors, SO ;

Jahic, M ;

Rozkov, A ;

Xu, B ;

Hecker, M ;

Jürgen, B ;

Krüger, E ;

Schweder, T ;

Hamer, G ;

O'Beirne, D ;

Noisommit-Rizzi, N ;

Reuss, M ;

Boone, L ;

Hewitt, C ;

McFarlane, C ;

Nienow, A ;

Kovacs, T ;

Trägårdh, C ;

Fuchs, L ;

Revstedt, J ;

Friberg, PC ;

Hjertager, B ;

Blomsten, G ;

Skogman, H ;

Hjort, S ;

Hoeks, F ;

Lin, HY ;

Neubauer, P ;

van der Lans, R ;

Luyben, K ;

Vrabel, P ;

Manelius, Å .

JOURNAL OF BIOTECHNOLOGY, 2001, 85 (02) :175-185

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