Rapid identification of health care-associated infections with an integrated fluorescence anisotropy system

被引:52
作者
Park, Ki Soo [1 ,2 ,3 ]
Huang, Chen-Han [1 ,2 ,3 ]
Lee, Kyungheon [1 ,2 ,3 ]
Yoo, Yeong-Eun [4 ]
Castro, Cesar M. [1 ,2 ,5 ]
Weissleder, Ralph [1 ,2 ,3 ,6 ]
Lee, Hakho [1 ,2 ,3 ]
机构
[1] Massachusetts Gen Hosp, Ctr Syst Biol, Boston, MA 02114 USA
[2] Harvard Med Sch, Boston, MA 02114 USA
[3] Harvard Med Sch, Dept Radiol, Boston, MA 02114 USA
[4] Korea Inst Machinery & Mat, Dept Nanomfg Technol, Daejeon 305343, South Korea
[5] Harvard Med Sch, Dept Med, Boston, MA 02114 USA
[6] Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA
基金
新加坡国家研究基金会;
关键词
RESISTANT STAPHYLOCOCCUS-AUREUS; VALENTINE LEUKOCIDIN GENES; URACIL-DNA-GLYCOSYLASE; MULTIPLEX PCR ASSAY; CARRYOVER CONTAMINATION; DISCRIMINATION; DIAGNOSIS; MECA;
D O I
10.1126/sciadv.1600300
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Health care-associated infections (HAIs) and drug-resistant pathogens have become a major health care issue with millions of reported cases every year. Advanced diagnostics would allow clinicians to more quickly determine the most effective treatment, reduce the nonspecific use of broad-spectrum antimicrobials, and facilitate enrollment in new antibiotic treatments. We present a new integrated system, polarization anisotropy diagnostics (PAD), for rapid detection of HAI pathogens. The PAD uses changes of fluorescence anisotropy when detection probes recognize target bacterial nucleic acids. The technology is inherently robust against environmental noise and economically scalable for parallel measurements. The assay is fast (2 hours) and performed on-site in a single-tube format. When applied to clinical samples obtained from interventional procedures, the PAD determined the overall bacterial burden, differentiated HAI bacterial species, and identified drug resistance and virulence status. The PAD system holds promise as a powerful tool for near-patient, rapid HAI testing.
引用
收藏
页数:9
相关论文
共 30 条
[1]   Burden of endemic health-care-associated infection in developing countries: systematic review and meta-analysis [J].
Allegranzi, Benedetta ;
Nejad, Sepideh Bagheri ;
Combescure, Christophe ;
Graafmans, Wilco ;
Attar, Homo ;
Donaldson, Liam ;
Pittet, Didier .
LANCET, 2011, 377 (9761) :228-241
[2]  
Aslanzadeh J, 2004, ANN CLIN LAB SCI, V34, P389
[3]   False-positive results and contamination in nucleic acid amplification assays: Suggestions for a prevent and destroy strategy [J].
Borst, A ;
Box, ATA ;
Fluit, AC .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2004, 23 (04) :289-299
[4]   Investigations on four host response factors whose expression is enhanced in X4 SHIV encephalitis [J].
Buch, S ;
Sui, YJ ;
Dhillon, N ;
Potula, R ;
Zien, C ;
Pinson, D ;
Li, SP ;
Dhillon, S ;
Nicolay, B ;
Sidelnik, A ;
Li, CC ;
Villinger, T ;
Bisarriya, K ;
Narayan, O .
JOURNAL OF NEUROIMMUNOLOGY, 2004, 157 (1-2) :71-80
[5]  
Chung HJ, 2013, NAT NANOTECHNOL, V8, P369, DOI [10.1038/NNANO.2013.70, 10.1038/nnano.2013.70]
[6]   The impact of hospital-acquired infections with multidrug-resistant bacteria in an oncology intensive care unit [J].
Cornejo-Juarez, P. ;
Vilar-Compte, D. ;
Perez-Jimenez, C. ;
Namendys-Silva, S. A. ;
Sandoval-Hernandez, S. ;
Volkow-Fernandez, P. .
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2015, 31 :31-34
[7]   A DNA-based machine that can cyclically bind and release thrombin [J].
Dittmer, WU ;
Reuter, A ;
Simmel, FC .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (27) :3550-3553
[8]   Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP) [J].
Hsieh, Kuangwen ;
Mage, Peter L. ;
Csordas, Andrew T. ;
Eisenstein, Michael ;
Soh, H. Tom .
CHEMICAL COMMUNICATIONS, 2014, 50 (28) :3747-3749
[9]   Real-time polymerase chain reaction for detecting bacterial DNA directly from blood of neonates being evaluated for sepsis [J].
Jordan, JA ;
Durso, MB .
JOURNAL OF MOLECULAR DIAGNOSTICS, 2005, 7 (05) :575-581
[10]   Automated Surveillance of Health Care-Associated Infections [J].
Klompas, Michael ;
Yokoe, Deborah S. .
CLINICAL INFECTIOUS DISEASES, 2009, 48 (09) :1268-1275