Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex

被引：44

作者：

Hussain, Tabish ^{[1
]}

Saha, Dhurjhoti ^{[1
,3
]}

Purohit, Gunjan ^{[1
,3
]}

Kar, Anirban ^{[1
]}

Mukherjee, Anand Kishore ^{[1
,3
]}

Sharma, Shalu ^{[1
,3
]}

Sengupta, Suman ^{[1
]}

Dhapola, Parashar ^{[2
,3
]}

Maji, Basudeb ^{[4
,5
]}

Vedagopuram, Sreekanth ^{[6
]}

Horikoshi, Nobuko T. ^{[7
]}

Horikoshi, Nobuo ^{[7
]}

Pandita, Raj K. ^{[7
]}

Bhattacharya, Santanu ^{[4
,5
]}

Bajaj, Avinash ^{[6
]}

Riou, Jean-Francois ^{[8
]}

Pandita, Tej K. ^{[7
]}

Chowdhury, Shantanu ^{[1
,2
,3
]}

机构：

[1] CSIR, Inst Genom & Integrat Biol, Genom & Mol Med Unit, Mathura Rd, New Delhi 110025, India

[2] CSIR, Inst Genom & Integrat Biol, GNR Knowledge Ctr Genome Informat, Mathura Rd, New Delhi 110025, India

[3] CSIR, Inst Genom & Integrat Biol, Acad Sci & Innovat Res AcSIR, Mathura Rd, New Delhi 110025, India

[4] Indian Inst Sci, Dept Organ Chem, Bangalore, Karnataka, India

[5] Jawaharlal Nehru Ctr Adv Sci Res, Chem Biol Unit, Bangalore 560012, Karnataka, India

[6] NCR Biotech Cluster, Reg Ctr Biotechnol, Lab Nanotechnol & Chem Biol, Faridabad 121001, Haryana, India

[7] Houston Methodist Res Inst, Dept Radiat Oncol, Houston, TX 77030 USA

[8] Museum Natl Hist Nat, Struct Acides Nucl Telomeres & Evolut, 43 Rue Cuvier, F-75231 Paris 05, France

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

英国惠康基金;

关键词：

TELOMERIC PROTEIN; QUADRUPLEX DNA; HUMAN RAP1; CELLS; SENESCENCE; APOPTOSIS; PROMOTER; CANCER; ATM; P53;

D O I：

10.1038/s41598-017-11177-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We observed extra-telomeric binding of the telomere repeat binding factor TRF2 within the promoter of the cyclin-dependent kinase CDKNIA (p21/CIP1/WAF1). This result in TRF2 induced transcription repression of p21. Interestingly, p21 repression was through engagement of the REST-coREST-LSD1-repressor complex and altered histone marks at the p21 promoter in a TRF2-dependent fashion. Furthermore, mutational analysis shows p21 repression requires interaction of TRF2 with a p21 promoter G-quadruplex. Physiologically, TRF2-mediated p21 repression attenuated drug-induced activation of cellular DNA damage response by evading G2/M arrest in cancer cells. Together these reveal for the first time role of TRF2 in REST-repressor complex mediated transcription repression.

引用

页数：13

共 59 条

[1] p21 in cancer: intricate networks and multiple activities [J].

Abbas, Tarek ;

Dutta, Anindya .

NATURE REVIEWS CANCER, 2009, 9 (06) :400-414

[2] TRF2 inhibition triggers apoptosis and reduces tumourigenicity of human melanoma cells [J].

Biroccio, Annamaria ;

Rizzo, Angela ;

Elli, Raffaella ;

Koering, Catherine-Elaine ;

Belleville, Aurelie ;

Benassi, Barbara ;

Leonetti, Carlo ;

Stevens, Malcolm F. G. ;

D'Incalci, Maurizio ;

Zupi, Gabriella ;

Gilson, Eric .

EUROPEAN JOURNAL OF CANCER, 2006, 42 (12) :1881-1888

[3] TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells [J].

Biroccio, Annamaria ;

Cherfils-Vicini, Julien ;

Augereau, Adeline ;

Pinte, Sebastien ;

Bauwens, Serge ;

Ye, Jing ;

Simonet, Thomas ;

Horard, Beatrice ;

Jamet, Karine ;

Cervera, Ludovic ;

Mendez-Bermudez, Aaron ;

Poncet, Delphine ;

Grataroli, Renee ;

de Rodenbeeke, Claire T'kint ;

Salvati, Erica ;

Rizzo, Angela ;

Zizza, Pasquale ;

Ricoul, Michelle ;

Cognet, Celine ;

Kuilman, Thomas ;

Duret, Helene ;

Lepinasse, Florian ;

Marvel, Jacqueline ;

Verhoeyen, Els ;

Cosset, Francois-Loic ;

Peeper, Daniel ;

Smyth, Mark J. ;

Londono-Vallejo, Arturo ;

Sabatier, Laure ;

Picco, Vincent ;

Pages, Gilles ;

Scoazec, Jean-Yves ;

Stoppacciaro, Antonella ;

Leonetti, Carlo ;

Vivier, Eric ;

Gilson, Eric .

NATURE CELL BIOLOGY, 2013, 15 (07) :818-+

[4] TELOMERES - NO END IN SIGHT [J].

BLACKBURN, EH .

CELL, 1994, 77 (05) :621-623

[5] Telomerase abrogation dramatically accelerates TRF2-induced epithelial carcinogenesis [J].

Blanco, Raquel ;

Munoz, Purificacion ;

Flores, Juana M. ;

Klatt, Peter ;

Blasco, Maria A. .

GENES & DEVELOPMENT, 2007, 21 (02) :206-220

[6] Multiple roles of the cell cycle inhibitor p21CDKN1A in the DNA damage response [J].

Cazzalini, Ornella ;

Scovassi, A. Ivana ;

Savio, Monica ;

Stivala, Lucia A. ;

Prosperi, Ennio .

MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2010, 704 (1-3) :12-20

[7] The Telomere Deprotection Response Is Functionally Distinct from the Genomic DNA Damage Response [J].

Cesare, Anthony J. ;

Hayashi, Makoto T. ;

Crabbe, Laure ;

Karlseder, Jan .

MOLECULAR CELL, 2013, 51 (02) :141-155

[8] The KRAS Promoter Responds to Myc-associated Zinc Finger and Poly(ADP-ribose) Polymerase 1 Proteins, Which Recognize a Critical Quadruplex-forming GA-element [J].

Cogoi, Susanna ;

Paramasivam, Manikandan ;

Membrino, Alexandro ;

Yokoyama, Kazunari K. ;

Xodo, Luigi E. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (29) :22003-22016

[9] Lack of TRF2 in ALT cells causes PML-dependent p53 activation and loss of telomeric DNA [J].

D'Alcontres, Martina Stagno ;

Mendez-Bermudez, Aaron ;

Foxon, Jennifer L. ;

Royle, Nicola J. ;

Salomoni, Paolo .

JOURNAL OF CELL BIOLOGY, 2007, 179 (05) :855-867

[10] Shelterin: the protein complex that shapes and safeguards human telomeres [J].

de Lange, T .

GENES & DEVELOPMENT, 2005, 19 (18) :2100-2110

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