An enterovirus strain isolated from diabetic child belongs to a genetic subcluster of echovirus 11, but is also neutralised with monotypic antisera to coxsackievirus A9

被引:31
作者
Al-Hello, Haider [2 ]
Paananen, Anja [2 ]
Eskelinen, Mervi [2 ]
Ylipaasto, Petri [2 ]
Hovi, Tapani [2 ]
Salmela, K. [1 ]
Lukashev, Alexander N. [3 ]
Bobegamage, Shubhada [4 ]
Roivainen, Merja [2 ]
机构
[1] Helsinki Univ Hosp, Renal Transplant Unit, Helsinki, Finland
[2] Natl Publ Hlth Inst, Enterovirus Lab, Helsinki, Finland
[3] Russian Acad Med Sci, Inst Poliomyelitis & Viral Encephalitis, Moscow 142782, Russia
[4] Slovak Med Univ, Dept Virol, Bratislava, Slovakia
关键词
D O I
10.1099/vir.0.83474-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An enterovirus strain (designated D207) isolated from a Slovakian diabetic child and originally serotyped as coxsackievirus A9 (CAV-9) was found to cause rapid cytolysis coinciding with severe functional damage of the surviving cells in primary cultures of human pancreatic islets. This finding prompted us to clone the isolate for full-length genome sequencing and molecular characterization as the prototype strain of CAV-9 is known to cause only minimal damage to insulin-producing fl-cells. Based on capsid-coding sequence comparisons, the isolate turned out to be echovirus 11 (E-1 1). Phylogenetic analyses demonstrated that E-1 1 /D207 was closely related to a specific subgroup B of E-1 1 strains known to cause uveitis. To study further antigenic properties of isolate E-1 1 /D207 and uveitis-causing E-1 1 strains, neutralization experiments were carried out with CAV-9- and E-1 1 -specific antisera. Unlike the prototype strains, the isolate E-1 1 /D207 and uveitis-causing E-1 1 strains were well neutralized with both CAV-9- and E-1 1 specific antisera. Attempts to identify recombination of the capsid coding sequences as a reason for double-reactivity using the Simplot analysis failed to reveal major transferred motifs. However, pepticle scanning technique was able to identify antigenic regions of capsid proteins of E-11 1 /D207 as well as regions cross-reacting with an antiserurn raised to CAV-9. Thus, double specificity of E- 11 /D207 seems to be a real characteristic shared by the phylogenetically closely related virus strains in the genetic subgroup B of E-1 1.
引用
收藏
页码:1949 / 1959
页数:11
相关论文
共 39 条
  • [31] 74::AID-JMV12&gt
  • [32] 3.0.CO
  • [33] 2-W
  • [34] Mechanisms of coxsackievirus-induced damage to human pancreatic β-cells
    Roivainen, M
    Rasilainen, S
    Ylipaasto, P
    Nissinen, R
    Ustinov, J
    Bouwens, L
    Eizirik, DL
    Hovi, T
    Otonkoski, T
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2000, 85 (01) : 432 - 440
  • [35] Determination of the structure of a decay accelerating factor-binding clinical isolate of echovirus 11 allows mapping of mutants with altered receptor requirements for infection
    Stuart, AD
    McKee, TA
    Williams, PA
    Harley, C
    Shen, S
    Stuart, DI
    Brown, TDK
    Lea, SM
    [J]. JOURNAL OF VIROLOGY, 2002, 76 (15) : 7694 - 7704
  • [36] The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools
    Thompson, JD
    Gibson, TJ
    Plewniak, F
    Jeanmougin, F
    Higgins, DG
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (24) : 4876 - 4882
  • [37] COMPLETE NUCLEOTIDE-SEQUENCE OF A BETA-CELL TROPIC VARIANT OF COXSACKIEVIRUS-B4
    TITCHENER, PA
    JENKINS, O
    SZOPA, TM
    TAYLOR, KW
    ALMOND, JW
    [J]. JOURNAL OF MEDICAL VIROLOGY, 1994, 42 (04) : 369 - 373
  • [38] Molecular analysis of an echovirus 3 strain isolated from an individual concurrently with appearance of islet cell and IA-2 autoantibodies
    Williams, CH
    Oikarinen, S
    Tauriainen, S
    Salminen, K
    Hyöty, H
    Stanway, G
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (02) : 441 - 448
  • [39] Global profiling of coxsackievirus- and cytokine-induced gene expression in human pancreatic islets
    Ylipaasto, P
    Kutlu, B
    Rasilainen, S
    Rasschaert, J
    Salmela, K
    Teerijoki, H
    Korsgren, O
    Lahesmaa, R
    Hovi, T
    Eizirik, DL
    Otonkoski, T
    Roivainen, M
    [J]. DIABETOLOGIA, 2005, 48 (08) : 1510 - 1522