Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and chikungunya viruses in clinical samples

被引：23
作者：
Mishra, Baijayantimala [1 ]
Sharma, Mirnalini [1 ]
Pujhari, Sujit Kumar [1 ]
Ratho, Radha Kanta [1 ]
Gopal, D. V. R. Sai [2 ]
Kumar, C. V. M. Naresh [2 ]
Sarangi, Gita [3 ]
Chayani, Nirupama [3 ]
Varma, Subhash C. [4 ]
机构：
[1] Post Grad Inst Med Educ & Res, Dept Virol, Chandigarh, India
[2] Sri Venkateshwara Univ Tirupati, Dept Virol, Tirupati, Andhra Pradesh, India
[3] SCB Med Coll, Dept Microbiol, Cuttack, Orissa, India
[4] Post Grad Inst Med Educ & Res, Dept Internal Med, Chandigarh, India
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2011年 / 71卷 / 02期
关键词：
Multiplex RT-PCR; Dengue; Chikungunya; Diagnosis; Serotyping; DISEASE; CLUSTAL;
D O I：
10.1016/j.diagmicrobio.2011.06.020
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The reemergence of chikungunya virus (CHIKV) has compounded the already existing dengue problem because of clinical similarities and common vector, demanding the need for a rapid and specific diagnosis. Thus, dengue chikungunya multiplex reverse transcriptase-polymerase chain reaction (DCmRT-PCR) was developed and validated for simultaneous detection of dengue and chikungunya viral infections and its utility in virus serotyping. Blood samples from 97 suspected dengue and chikungunya cases and 10 healthy controls were subjected to dengue and chikungunya conventional RT-PCR and DCmRT-PCR. Thirty-one of 97 samples were positive for dengue or chikungunya viral RNA by RT-PCR and DCmRT-PCR with 100% concordance. DCmRT-PCR products were cycle sequenced. Seven dengue virus strains were clustered within genotype III of DENV-3 and 4 within genotype III of DENV-1, whereas chikungunya sequences were clustered within the Central/East African genotype. DCmRT-PCR was found to be a potential rapid test for simultaneous detection of dengue and CHIKV in clinical samples along with dengue serotyping. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：118 / 125
页数：8
相关论文
共 24 条
  • [1] Reemergence of dengue virus type-3 (subtype-III) in India:: Implications for increased incidence of DHF & DSS
    Dash, Paban Kumar
    Parida, Man Mohan
    Saxena, Parag
    Abhyankar, Ajay
    Singh, C. P.
    Tewari, K. N.
    Jana, Asha Mukul
    Sekhar, K.
    Rao, P. V. Lakshmana
    [J]. VIROLOGY JOURNAL, 2006, 3 (1)
  • [2] One-step RT-PCR protocols improve the rate of dengue diagnosis compared to two-step RT-PCR approaches
    De Paula, SO
    De Melo Lima, C
    Torres, MP
    Pereira, MRG
    da Fonseca, BAL
    [J]. JOURNAL OF CLINICAL VIROLOGY, 2004, 30 (04) : 297 - 301
  • [3] Dengue and dengue hemorrhagic fever
    Gubler, DJ
    [J]. CLINICAL MICROBIOLOGY REVIEWS, 1998, 11 (03) : 480 - +
  • [4] The changing epidemiology of dengue in Delhi, India
    Gupta, Ekta
    Dar, Lalit
    Kapoor, Geetanjali
    Broor, Shobha
    [J]. VIROLOGY JOURNAL, 2006, 3 (1)
  • [5] Higgins DG, 1996, METHOD ENZYMOL, V266, P383
  • [6] Chikungunya disease in nonhuman primates involves long-term viral persistence in macrophages
    Labadie, Karine
    Larcher, Thibaut
    Joubert, Christophe
    Mannioui, Abdekrim
    Delache, Benoit
    Brochard, Patricia
    Guigand, Lydie
    Dubreil, Laurence
    Lebon, Pierre
    Verrier, Bernard
    de lamballerie, Xavier
    Suhrbier, Andreas
    Cherel, Yan
    Le Grand, Roger
    Roques, Pierre
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2010, 120 (03) : 894 - 906
  • [7] RAPID DETECTION AND TYPING OF DENGUE VIRUSES FROM CLINICAL-SAMPLES BY USING REVERSE TRANSCRIPTASE-POLYMERASE CHAIN-REACTION
    LANCIOTTI, RS
    CALISHER, CH
    GUBLER, DJ
    CHANG, GJ
    VORNDAM, AV
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (03) : 545 - 551
  • [8] Clustal W and clustal X version 2.0
    Larkin, M. A.
    Blackshields, G.
    Brown, N. P.
    Chenna, R.
    McGettigan, P. A.
    McWilliam, H.
    Valentin, F.
    Wallace, I. M.
    Wilm, A.
    Lopez, R.
    Thompson, J. D.
    Gibson, T. J.
    Higgins, D. G.
    [J]. BIOINFORMATICS, 2007, 23 (21) : 2947 - 2948
  • [9] Clinical Applicability of Single-Tube Multiplex Reverse-Transcriptase PCR in Dengue Virus Diagnosis and Serotyping
    Mishra, Bajayantimala
    Sharma, Mirnalini
    Pujhari, Sujit Kumar
    Appannanavar, Suma B.
    Ratho, Radha Kanta
    [J]. JOURNAL OF CLINICAL LABORATORY ANALYSIS, 2011, 25 (02) : 76 - 78
  • [10] Mourya DT, 2001, ACTA VIROL, V45, P305
123