Data mining for miRNAs and their targets in the Triticeae

被引:43
作者
Dryanova, Ani [1 ]
Zakharov, Arseny [2 ]
Gulick, Patrick J. [1 ]
机构
[1] Concordia Univ, Dept Biol, Montreal, PQ H4B 1R6, Canada
[2] Concordia Univ, Dept Math, Montreal, PQ H4B 1R6, Canada
关键词
microRNA; miRNA; data mining; miRNA precursor; miRNA target; Triticeae; wheat; barley;
D O I
10.1139/G08-025
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
MicroRNAs (miRNAs) and the mRNA targets of miRNAs were identified by sequence complementarity within a DNA sequence database for species of the Triticeae. Data screening identified 28 miRNA precursor sequences from 15 miRNA families that contained conserved mature miRNA sequences within predicted stem-loop structures. In addition, the identification of 337 target sequences among Triticeae genes provided further evidence of the existence of 26 miRNA families in the cereals. MicroRNA targets included genes that are homologous to known targets in diverse model species as well as novel targets. MicroRNA precursors and targets were identified in 10 related species, though the great majority of them were identified in bread wheat, Triticum aestivum, and barley, Hordeum vulgare, the two species with the largest EST data sets among the Triticeae.
引用
收藏
页码:433 / 443
页数:11
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