Bitter taste receptors protect against skin aging by inhibiting cellular senescence and enhancing wound healing

被引：11
作者：
Chung, Min Gi [1 ]
Kim, Yerin [1 ]
Cha, Yeon Kyung [2 ]
Park, Tai Hyun [2 ,3 ]
Kim, Yuri [1 ]
机构：
[1] Ewha Womans Univ, Dept Nutr Sci & Food Management, 52 Ewhayeodae Gil, Seoul 03760, South Korea
[2] Seoul Natl Univ, Interdisciplinary Program Bioengn, Seoul 08826, South Korea
[3] Seoul Natl Univ, Sch Chem & Biol Engn, Inst Chem Proc, Seoul 08826, South Korea
来源：
NUTRITION RESEARCH AND PRACTICE | 2022年 / 16卷 / 01期
关键词：
Taste; galactose; skin aging; wound healing; keratinocytes; D-GALACTOSE; DERMAL FIBROBLASTS; IN-VITRO; MOUSE; ALOIN;
D O I：
10.4162/nrp.2022.16.1.1
中图分类号：
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号：
100403 ;
摘要：
BACKGROUND/OBJECTIVES: Bitter taste receptors are taste signaling pathway mediators, and are also expressed and function in extra-gustatory organs. Skin aging affects the quality of life and may lead to medical issues. The purpose of this study was to better understand the anti-skin aging effects of bitter taste receptors in D-galactose (D-gal)-induced aged human keratinocytes, HaCaT cells. MATERIALS/METHODS: Expressions of bitter taste receptors in HaCaT cells and mouse skin tissues were examined by polymerase chain reaction assay. Bitter taste receptor was overexpressed in HaCaT cells, and D-gal was treated to induce aging. We examined the effects of bitter taste receptors on aging by using beta-galactosidase assay, wound healing assay, and Western blot assay. RESULTS: TAS2R16 and TAS2R10 were expressed in HaCaT cells and were upregulated by D-gal treatment. TAS2R16 exerted protective effects against skin aging by regulating p53 and p21, antioxidant enzymes, the SIRT1/mechanistic target of rapamycin pathway, cell migration, and epithelial-mesenchymal transition markers. TAS2R10 was further examined to confirm a role of TAS2R16 in cellular senescence and wound healing in ll-gal-induced aged HaCaT cells. CONCLUSIONS: Our results suggest a novel potential preventive role of these receptors on skin aging by regulating cellular senescence and wound healing in human keratinocyte, HaCaT.
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页码：1 / 13
页数：13
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