Magnetic purification of plasminogen from human plasma by specific lysine affinity

被引：9

作者：

Liu, Chi-Hsien ^{[1
]}

Wu, Wei-Chi ^{[2
,3
]}

Lai, Hsuan-Yu ^{[1
]}

Hou, Hau-Yaun ^{[1
]}

机构：

[1] Chang Gang Univ, Grad Inst Biochem & Biomed Engn, Tao Yuan 333, Taiwan

[2] Chang Gang Univ, Coll Med, Tao Yuan 333, Taiwan

[3] Chang Gung Mem Hosp, Dept Ophthalmol, Tao Yuan, Taiwan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2011年 / 112卷 / 03期

关键词：

Magnetic purification; Plasminogen; Plasma; Lysine; Nanoparticles; MESOPOROUS SILICA; NANO-ADSORBENT; ADSORPTION; BINDING; PROTEIN; DEHYDROGENASE; KINETICS; REMOVAL; SYSTEM; LIPASE;

D O I：

10.1016/j.jbiosc.2011.05.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel magnetic adsorbent was prepared by covalently binding lysine onto the surface of nanoparticles via a carbodiimide coupling method. The adsorption of plasminogen onto surface-modified magnetic nanoparticles from human plasma was studied in a batch system. Surface modifications of particles were characterized using Fourier-transformed infrared spectra, transmission electron micrography, and ninhydrin assay. The maximum weight ratio of lysine to the superparamagnetic particles was 30 mu mol/mg of particles. Effects of pH and temperature on plasminogen adsorption by the magnetic particles were evaluated. Desorption of plasminogen from the magnetic adsorbent was investigated using aminocaproic acid, a lysine analogue. Using a buffer composed by aminocaproic acid or lysine, plasminogen on the magnetic nanoparticles could be eluted. Overall, the results demonstrated that the lysine-coated magnetic adsorbent increased the efficiency and speed of recovery of plasminogen from human plasma. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：219 / 224

页数：6

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