Expression and In Vitro Function of Anti-Breast Cancer Llama-Based Single Domain Antibody VHH Expressed in Tobacco Plants

被引:24
作者
Park, Se Ra [1 ]
Lee, Jeong-Hwan [1 ]
Kim, Kibum [1 ,2 ]
Kim, Taek Min [3 ]
Lee, Seung Ho [3 ]
Choo, Young-Kug [4 ]
Kim, Kyung Soo [2 ]
Ko, Kisung [1 ]
机构
[1] Chung Ang Univ, Coll Med, Med Res Inst, Dept Med, Seoul 156756, South Korea
[2] Chung Ang Univ, Dept Otorhinolaryngol Head & Neck Surg, Coll Med, Seoul 156756, South Korea
[3] Incheon Natl Univ, Coll Life Sci & Bioengn, Nanobioengn, Incheon 22012, South Korea
[4] Wonkwang Univ, Coll Nat Sci, Dept Biol Sci, 460 Iksan Daero, Iksan Si 54538, Jeollabuk Do, South Korea
基金
新加坡国家研究基金会;
关键词
breast cancer; HER2; Herceptin; VHH antibody; plant antibody; MONOCLONAL-ANTIBODY; PROTEIN; GLYCANS; RECEPTORS; VACCINES;
D O I
10.3390/ijms21041354
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Overexpression of human epidermal growth factor receptor type 2 (HER2) is considered as a prognostic factor of breast cancer, which is positively associated with recurrence when cancer metastasizes to the lymph nodes. Here, we expressed the single variable domain on a heavy chain (VHH) form of anti-HER2 camelid single domain antibody in tobacco plants and compared its in vitro anticancer activities with the anti-HER2 full size antibody. The gene expression cassette containing anti-HER2 camelid single domain antibody VHH fused to human IgG Fc region with KDEL endoplasmic reticulum (ER) (VHH-FcK) was transferred into the tobacco plant via the Agrobacterium-mediated transformation. The transformants were screened with polymerase chain reaction and Western blot analyses. Enzyme-linked immunosorbent assay (ELISA) confirmed the binding of the purified anti-HER2 VHH-FcK to the HER2-positive breast cancer cell line, SK-BR-3. Migration assay results confirmed anticancer activity of the plant-derived anticancer camelid single chain antibody. Taken together, we confirmed the possibility of using anti-HER2 VHH-FcK as a therapeutic anticancer agent, which can be expressed and assembled and purified from a plant expression system as an alternative antibody production system.
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页数:12
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