Evaluation of resistant genotypes and their characterization using molecular markers linked for powdery mildew resistance in cucumber (Cucumis sativus L.)

被引：4
作者：
Sharma, Susheel [1 ]
Dar, Aejaz Ahmad [1 ]
Gupta, Sachin [2 ]
Singh, Ravinder [1 ]
机构：
[1] Sher e Kashmir Univ Agr Sci & Technol Jammu SKUAS, Sch Biotechnol, Chatha, Jammu & Kashmir, India
[2] Sher e Kashmir Univ Agr Sci & Technol Jammu SKUAS, Div Plant Pathol, Chatha 180009, Jammu, India
来源：
PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION | 2021年 / 19卷 / 06期
关键词：
breeding; cucumber; disease resistance; molecular markers; powdery mildew; QTL; LOCI;
D O I：
10.1017/S1479262121000605
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Powdery mildew (PM) is one of the most severe fungal diseases of cucumber that limits its production worldwide. In this study, 140 genotypes of cucumber were screened for disease resistance under field and artificial conditions, and then validated with eight known SSR markers linked to PM resistance. Among these genotypes, genotype GS140 was found resistant (R), whereas GS148, GS16 and GS70 were moderately resistant, and GS169 was found to be tolerant. Of all the eight markers, only C31, C80, C162, SSR16472 and SSR16881 amplified the reported linked allele. The 127 bp allele of SSR16881 was found to be associated with the lowest disease severity of 37.65%. The associated markers could further be verified for their usability using linkage studies and the contrast genotypes in the present study could serve as a tool for selection in future marker-assisted selection breeding strategies for PM resistance.
引用
收藏
页码：497 / 502
页数：6
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