Screening for serological biomarkers of pancreatic cancer by two-dimensional electrophoresis and liquid chromatography-tandem mass spectrometry

被引：14

作者：

Wang, Yufeng ^{[1
]}

Kuramitsu, Yasuhiro ^{[1
]}

Yoshino, Shigefumi ^{[2
]}

Takashima, Motonari ^{[2
]}

Zhang, Xiulian ^{[1
]}

Ueno, Tomio ^{[2
]}

Suzuki, Nobuaki ^{[2
]}

Oka, Masaaki ^{[2
]}

Nakamura, Kazuyuki ^{[1
]}

机构：

[1] Yamaguchi Univ, Grad Sch Med, Dept Biochem & Funct Prote, Ube, Yamaguchi 7558505, Japan

[2] Yamaguchi Univ, Grad Sch Med, Dept Digest Surg Appl Mol Biosci, Ube, Yamaguchi 7558505, Japan

来源：

ONCOLOGY REPORTS | 2011年 / 26卷 / 01期

关键词：

two-dimensional gel electrophoresis; liquid chromatography-tandem mass spectrometry; pancreatic cancer; HEPATITIS-C-VIRUS; PROTEOMIC ANALYSIS; HEPATOCELLULAR-CARCINOMA; PROSTATE-CANCER; CELL CARCINOMA; BREAST-CANCER; SERUM; ALPHA-1-ANTITRYPSIN; IDENTIFICATION; OVEREXPRESSION;

D O I：

10.3892/or.2011.1278

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Pancreatic cancer (PC) is one of the most lethal. malignant tumors because of late diagnosis and the lack of response to various therapies. To identify potential biomarkers in cancerous serum from early stage PC patients, we carried out two-dimensional gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to compare the serum proteomic profiles from 45 patients with PC and 20 healthy volunteers. Seven spots showed differential expression on 2-DE gels and two up-regulated protein spots were identified by LC-MS/MS as alpha-1-antitrypsin (AAT). These protein spots were also confirmed by Western blotting. This is the first time that AAT isoforms have been identified as potential serum biomarkers for PC. The serum isoforms of AAT may be clinically useful for PC diagnosis and monitoring.

引用

页码：287 / 292

页数：6

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