MicroRNA-184 inhibits cell proliferation and invasion, and specifically targets TNFAIP2 in Glioma

被引：71

作者：

Cheng, Zhe ^{[1
]}

Wang, Hang Zhou ^{[1
]}

Li, Xuetao ^{[1
]}

Wu, Zhiwu ^{[1
]}

Han, Yong ^{[1
]}

Li, Yanyan ^{[1
]}

Chen, Guilin ^{[1
]}

Xie, Xueshun ^{[1
]}

Huang, Yulun ^{[1
]}

Du, Ziwei ^{[1
]}

Zhou, Youxin ^{[1
]}

机构：

[1] Soochow Univ, Affiliated Hosp 1, Neurosurg & Brain & Nerve Res Lab, Suzhou 215006, Jiangsu, Peoples R China

来源：

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH | 2015年 / 34卷

基金：

中国国家自然科学基金;

关键词：

miR-184; TNFAIP2; Proliferation; Invasion; Glioma; NASOPHARYNGEAL CARCINOMA; TUMOR-SUPPRESSOR; MIR-184; NEUROBLASTOMA; EXPRESSION; PROGNOSIS; APOPTOSIS; SURVIVAL; GENE; DIFFERENTIATION;

D O I：

10.1186/s13046-015-0142-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: miRNA-184 is an oncogene in human hepatocellular carcinoma but acts as a tumor suppressor in tongue squamous cell carcinoma. Studies have shown that miR-184 was down-regulated in glioma and TNF alpha-induced protein 2 (TNFAIP2) was closely related to tumorigenesis. This study aimed to determine the functions of miR-184 in glioma and the mechanisms of miRNA-184-TNFAIP2 mediated glioma progression. Methods: Real-time reverse-transcription PCR detected expression of miR-184 and TNFAIP2. U87 and U251 cells were transfected with miR-184 mimic, inhibitor, or negative control miRNA, and their invasion abilities were assayed. Cellular proliferation was measured by the cell counting kit-8 assay. miR-184 effects on glioma cell apoptosis and cell cycle were assessed by flow cytometer. Biological information software have predicted that miR-184 could target TNFa-induced protein 2 (TNFAIP2), Which was further validated by Western blot and qRT-PCR in glioma cells. In vivo, U87 cells transduced with either lentiviral over-expressed miR-184 or control lentivirus were injected into nude mice subcutaneously and intracranial respectively. Results: Expression of miR-184 was significantly lower in glioma tissues and cell-lines compared to normal brain tissues. Protein and mRNA expression of TNFAIP2 were inversely correlated with miR-184 in glioma. In vitro, proliferation and invasion abilities were also decreased in U87 and U251 cells after transfection with miR-184 mimic. In vivo, the xenografted tumor size in the miR-184 overexpressing group were smaller than the miR-NC group. Concordantly, U87 and U251 cells transfected with miR-184 mimic had a higher apoptosis rate, triggering an accumulation of cells at the G0/G1 phase and decreased cells in S-phase. Conclusions: miR-184 could regulate TNFAIP2 expression and affected its translation in glioma. miR-184 could also inhibit glioma progression and might serve as a novel therapeutic target in glioma.

引用

页数：13

共 31 条

[1] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].