High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging

被引:272
作者
Turtaev, Sergey [1 ,2 ]
Leite, Ivo T. [1 ,3 ]
Altwegg-Boussac, Tristan [4 ]
Pakan, Janelle M. P. [4 ,5 ]
Rochefort, Nathalie L. [4 ,6 ]
Cizmar, Tomas [1 ,3 ,7 ]
机构
[1] Leibniz Inst Photon Technol, Albert Einstein Str 9, D-07745 Jena, Germany
[2] Univ Dundee, Sch Life Sci, Dundee DD1 4HN, Scotland
[3] Univ Dundee, Sch Sci & Engn, Dundee DD1 4HN, Scotland
[4] Univ Edinburgh, Ctr Discovery Brain Sci, Hugh Robson Bldg 15,George Sq, Edinburgh EH8 9XD, Midlothian, Scotland
[5] German Ctr Neurodegenerat Dis, Inst Cognit Neurol & Dementia Res, Ctr Behav Brain Sci, Leipziger Str 44,Haus 64, D-39120 Magdeburg, Germany
[6] Univ Edinburgh, Simons Initiat Developing Brain, Edinburgh EH8 9XD, Midlothian, Scotland
[7] Inst Sci Instruments CAS, Kralovopolska 147, Brno 61264, Czech Republic
基金
英国惠康基金; 欧洲研究理事会;
关键词
OPTICAL-FIBER; FOCUSING LIGHT; MEDIA; MODULATION; TRANSMISSION; AMPLITUDE; TISSUE; TIME;
D O I
10.1038/s41377-018-0094-x
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Progress in neuroscience relies on new techniques for investigating the complex dynamics of neuronal networks. An ongoing challenge is to achieve minimally invasive and high-resolution observations of neuronal activity in vivo inside deep brain areas. Recently introduced methods for holographic control of light propagation in complex media enable the use of a hair-thin multimode optical fibre as an ultranarrow imaging tool. Compared to endoscopes based on graded-index lenses or fibre bundles, this new approach offers a footprint reduction exceeding an order of magnitude, combined with a significant enhancement in resolution. We designed a compact and high-speed system for fluorescent imaging at the tip of a fibre, achieving a resolution of 1.18 +/- 0.04 mu m across a 50-mu m field of view, yielding 7-kilopixel images at a rate of 3.5 frames/s. Furthermore, we demonstrate in vivo observations of cell bodies and processes of inhibitory neurons within deep layers of the visual cortex and hippocampus of anaesthetised mice. This study paves the way for modern microscopy to be applied deep inside tissues of living animal models while exerting a minimal impact on their structural and functional properties.
引用
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页数:8
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