Identification of Purple Acid Phosphatases in Chickpea and Potential Roles of CaPAP7 in Seed Phytate Accumulation

被引：65

作者：

Bhadouria, Jyoti ^{[1
]}

Singh, Ajit Pal ^{[1
]}

Mehra, Poonam ^{[1
]}

Verma, Lokesh ^{[1
]}

Srivastawa, Rishi ^{[1
]}

Parida, Swarup K. ^{[1
]}

Giri, Jitender ^{[1
]}

机构：

[1] Natl Inst Plant Genome Res, Aruna Asaf Ali Marg, New Delhi 110067, India

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

MOLECULAR CHARACTERIZATION; ARABIDOPSIS-THALIANA; PHYTIC ACID; BIOCHEMICAL-CHARACTERIZATION; FUNCTIONAL-ANALYSIS; SWEET-POTATO; PHOSPHORUS; GENE; RICE; EXPRESSION;

D O I：

10.1038/s41598-017-11490-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Purple acid phosphatases (PAPs) play important roles in phosphate (Pi) acquisition and utilization. These PAPs hydrolyze organic Phosphorus (P) containing compounds in rhizosphere as well as inside the plant cell. However, roles of PAPs in one of the most widely cultivated legumes, chickpea (Cicer arietnum L.), have not been unraveled so far. In the present study, we identified 25 putative PAPs in chickpea (CaPAPs) which possess functional PAP motifs and domains. Differential regulation of CaPAPs under different nutrient deficiencies revealed their roles under multiple nutrient stresses including Pi deficiency. Interestingly, most of the CaPAPs were prominently expressed in flowers and young pods indicating their roles in flower and seed development. Association mapping of SNPs underlying CaPAPs with seed traits revealed significant association of low Pi inducible CaPAP7 with seed weight and phytate content. Biochemical characterization of recombinant CaPAP7 established it to be a functional acid phosphatase with highest activity on most abundant organic-P substrate, phytate. Exogenous application of recombinant CaPAP7 enhanced biomass and Pi content of Arabidopsis seedlings supplemented with phytate as sole P source. Taken together, our results uncover the PAPs in chickpea and potential roles of CaPAP7 in seed phytate accumulation.

引用

页数：12

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