MALDI-TOF MS for quality control of high protein content sport supplements

被引：15

作者：

De Ceglie, Cristina ^{[1
]}

Calvano, Cosima D. ^{[1
]}

Zambonin, Carlo G. ^{[1
,2
]}

机构：

[1] Univ Bari Aldo Moro, Dipartimento Chim, I-70126 Bari, Italy

[2] Univ Bari Aldo Moro, Ctr Interdipartimentale Ric SMART, I-70126 Bari, Italy

来源：

FOOD CHEMISTRY | 2015年 / 176卷

关键词：

Sport supplements; High quality proteins; MALDI; Egg; Milk; VIRGIN OLIVE OIL; AMINO-ACIDS; MASS-SPECTROMETRY; HAZELNUT OIL; MILK; IDENTIFICATION;

D O I：

10.1016/j.foodchem.2014.12.038

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

High protein content sport nutritional supplements are found as powder products containing, as ingredients, amino acids and proteins with important nutritional values as milk, soy and egg proteins. An EU Food Supplements Directive (2002) requires that supplements should be safe, both in dosages and in purity. It is important, then, to develop rapid and sensitive methods to be employed for the quality control of these substances. In this work, we apply, for the first time, matrix-assisted laser desorption ionization-mass spectrometry as a fast, reproducible and sensitive method for the quality control of sport nutritional supplements based on proteins. To this aim, several commercial egg- and/or milk-based powder products have been processed by in gel or in solution digestion and analyzed in comparison to pure standard products. This strategy allowed to assess the reliability of the indications on proteins (as caseins, whey proteins and ovalbumin) declared in the label of several sport nutritional supplements. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：396 / 402

页数：7

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