Comparative Study of Human Hematopoietic Cell Engraftment into Balb/c and C57BL/6 Strain of Rag-2/Jak3 Double-Deficient Mice

被引：71

作者：

Ono, Ayumi ^{[1
]}

Hattori, Shinichiro ^{[1
]}

Kariya, Ryusho ^{[1
]}

Iwanaga, Sumako ^{[1
]}

Taura, Manabu ^{[1
]}

Harada, Hideki ^{[1
]}

Suzu, Shinya ^{[1
]}

Okada, Seiji ^{[1
]}

机构：

[1] Kumamoto Univ, Ctr AIDS Res, Div Hematopoiesis, Kumamoto 8600811, Japan

来源：

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY | 2011年

关键词：

IMMUNE-SYSTEM; BLOOD; RECOMBINATION; LYMPHOCYTES; DEFECTS; MODEL; JAK3;

D O I：

10.1155/2011/539748

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Immunodeficient mice are becoming invaluable tools in human stem cell and tumor research. In this study, we generated Rag-2/Jak3 double-deficient (Rag-2(-/-)Jak3(-/-)) mice with a C57/BL6 and Balb/c genetic background and compared the human lymphohematopoietic cell engraftment rate. Human cord blood-derived CD34(+) hematopoietic stem cells were successfully engrafted into Balb/c Rag-2(-/-)Jak3(-/-) mice; however, the engraftment rate was far lower in C57/BL6 Rag-2(-/-)Jak3(-/-) mice. Transplantation of human peripheral blood mononuclear cells resulted in the same tendency. Thus, a Balb/c background offers superior engraftment capacity than a C57/BL6 background and provides an attractive model for human hematopoietic cell engraftment.

引用

页数：6

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