Comparison of real-time polymerase chain reaction and DNA-strip technology in microbiological evaluation of periodontitis treatment

被引：61

作者：

Eick, Sigrun ^{[1
,2
]}

Straube, Anna ^{[1
]}

Guentsch, Arndt ^{[3
]}

Pfister, Wolfgang ^{[1
]}

Jentsch, Holger ^{[4
]}

机构：

[1] Univ Hosp Jena, Inst Med Microbiol, D-07747 Jena, Germany

[2] Univ Bern, Dept Periodontol, Lab Oral Microbiol, CH-3010 Bern, Switzerland

[3] Univ Hosp Jena, Dept Conservat Dent, D-07743 Jena, Germany

[4] Univ Hosp Leipzig, Dept Conservat Dent & Periodontol, D-04103 Leipzig, Germany

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2011年 / 69卷 / 01期

关键词：

Periodontopathogenic bacteria; Real-time PCR; DNA-strip technology; SUBGINGIVAL PLAQUE; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; PORPHYROMONAS-GINGIVALIS; BACTEROIDES-FORSYTHUS; TANNERELLA-FORSYTHIA; TREPONEMA-DENTICOLA; PCR; CULTURE; CHECKERBOARD; IDENTIFICATION;

D O I：

10.1016/j.diagmicrobio.2010.08.017

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The impact of a semiquantitative commercially available test based on DNA-strip technology (microlDent (R), Hain Lifescience, Nehren, Germany) on diagnosis and treatment of severe chronic periodontitis of 25 periodontitis patients was evaluated in comparison with a quantitative in-house real-time PCR. Subgingival plaque samples were collected at baseline as well as at 3, 6, and 12 months later. After extracting DNA, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, and several other periodontopathogens were determined by both methods. The results obtained by DNA-strip technology were analyzed semiquantitatively and additionally quantitatively by densitometry. The results for the 4 major periodontopathogenic bacterial species correlated significantly between the 2 methods. Samples detecting a high bacterial load by one method and negative by the other were always found in less than 2% of the total samples. Both technologies showed the impact of treatment on microflora. Especially the semiquantitative DNA-strip technology clearly analyzed the different loads of periodontopathogens after therapy and is useful in microbial diagnostics for patients in dental practices. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：12 / 20

页数：9

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