Rapid detection of Salmonella enterica serovars by multiplex PCR

被引：6

作者：

Jeyasekaran, G. ^{[1
,2
]}

Raj, K. Thirumalai ^{[1
,2
]}

Shakila, R. Jeya ^{[1
,2
]}

Thangarani, A. Jemila ^{[1
,2
]}

Sukumar, D. ^{[1
,2
]}

Jailani, V. Abdul Kadhar ^{[1
,2
]}

机构：

[1] Tamil Nadu Vet & Anim Sci Univ, Dept Fish Proc Technol, Coll Fisheries, Tuticorin 628008, India

[2] Tamil Nadu Vet & Anim Sci Univ, Res Inst, Tuticorin 628008, India

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2011年 / 27卷 / 04期

关键词：

Multiplex PCR; Salmonella; V; cholerae; E; coli; Genus specific genes; POLYMERASE-CHAIN-REACTION; CONVENTIONAL CULTURE; GENE; IDENTIFICATION; TYPHIMURIUM; PATHOGENS; ASSAY; INVA; AMPLIFICATION; OPTIMIZATION;

D O I：

10.1007/s11274-010-0538-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A multiplex PCR based assay was developed for the identification of the genus Salmonella. Five sets of primers from different genomic sequences such as fimA, himA, hns, invA and hto genes were selected for the identification of serogroups of Salmonella enterica such as S. Typhi, S. ParatyphiA, S. Typhimurium, S. Enteritidis and S. Weltevreden. The selected primers amplified products with the sizes of 85, 123, 152, 275 and 496 bp, respectively, for the genus Salmonella. This assay was found to be highly sensitive, as it could detect 5 cells of Salmonella and 1,000 fg of genomic DNA. Amplification of DNA extracted from other genera viz. V. cholerae and E. coli yielded negative results. This assay provides specific and reliable results and allows for the cost-effective detection of Salmonella in one reaction tube in mixed bacterial communities.

引用

页码：953 / 959

页数：7

共 35 条

[1] SALMONELLA IDENTIFICATION BY THE POLYMERASE CHAIN-REACTION [J].