A new dehydrogenase from Clostridium acetobutylicum for asymmetric synthesis: dynamic reductive kinetic resolution entry into the Taxotere side chain

被引：42
作者：
Applegate, Gregory A.
Cheloha, Ross W.
Nelson, David L.
Berkowitz, David B. [1 ]
机构：
[1] Univ Nebraska, Dept Chem, Lincoln, NE 68588 USA
来源：
CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 08期
基金：
美国国家科学基金会;
关键词：
ENZYMATIC SCREENING ISES; BETA-AMINO ALCOHOLS; DIRECTED EVOLUTION; CHEMOENZYMATIC SYNTHESIS; SUBSTRATE-SPECIFICITY; EPOXIDE HYDROLASE; ALLYLIC AMINATION; BUILDING-BLOCKS; ATCC; 824; BUTANOL;
D O I：
10.1039/c0cc04585c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An NADP-dependent alcohol dehydrogenase from Clostridium acetobutylicum (CaADH) has been expressed and characterized. CaADH enantioselectively reduces aromatic alpha-, beta- and gamma-keto esters to the corresponding D-hydroxy esters and provides a building block for the Taxotere side chain (95% yield, 95% de, 99% ee) by dynamic reductive kinetic resolution (DYRKR).
引用
收藏
页码：2420 / 2422
页数：3
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