Molecular cloning of phospholipases A2 from venom glands of Echis carpet vipers

被引：20

作者：

Bharati, K ^{[1
]}

Hasson, SS ^{[1
]}

Oliver, J ^{[1
]}

Laing, GD ^{[1
]}

Theakston, RDG ^{[1
]}

Harrison, RA ^{[1
]}

机构：

[1] Univ Liverpool, Liverpool Sch Trop Med, Alistair Reid Venom Res Unit, Liverpool L3 5QA, Merseyside, England

来源：

TOXICON | 2003年 / 41卷 / 08期

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

phospholipases A(2); Echis pyramidum leakeyi; Echis sochureki; Echis ocellatus; cDNA cloning;

D O I：

10.1016/S0041-0101(02)00312-4

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Venom toxin-specific antibodies offer a more rational treatment of snake envenoming than conventional antivenom. Here, we describe novel cDNAs encoding phospholipase A(2) (PLA(2)) isoforms from venom gland RNA of Echis pyramidum leakeyi (Epl), Echis sochureki (Es) and Echis ocellatus (Eo). The deduced amino acid sequences of these cDNAs encoded proteins with high overall sequence identity to the viper group II PLA(2) protein family, including the 14 cysteine residues capable of forming seven disulphide bonds that characterize this group of PLA(2) enzymes. Comparison of the PLA(2) sequences from Echis with those from related vipers failed to make significant geographic, taxonomic or PLA(2)-function distinctions between these Echis PLA(2) isoforms. However, their deduced hydrophilicity profiles revealed a conserved tertiary structure that we will exploit, by epidermal DNA immunization, to generate PLA(2)-neutralizing antibodies with polyspecific potential. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：941 / 947

页数：7

共 24 条

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