Prevention of Candida albicans Biofilm Formation by Covalently Bound Dimethylaminoethylmethacrylate and Polyethylenimine

被引：38
作者：
De Prijck, Kristof [1 ]
De Smet, Nele [2 ]
Coenye, Tom [1 ]
Schacht, Etienne [2 ]
Nelis, Hans J. [1 ]
机构：
[1] Univ Ghent, Lab Pharmaceut Microbiol, Fac Pharmaceut Sci, B-9000 Ghent, Belgium
[2] Univ Ghent, Lab Polymer Chem & Biomat, B-9000 Ghent, Belgium
来源：
MYCOPATHOLOGIA | 2010年 / 170卷 / 04期
关键词：
Grafting; Polyethylenimine; Dimethylaminoethylmethacrylate; Candida albicans; Biofilm; Modified Robbins Device; BIOLOGICALLY-ACTIVE POLYMERS; AMMONIUM SILANE COATINGS; QUATERNARY AMMONIUM; ANTIMICROBIAL ACTIVITY; SURFACE MODIFICATION; VOICE PROSTHESES; IN-VITRO; REHABILITATION; IMMOBILIZATION; COLONIZATION;
D O I：
10.1007/s11046-010-9316-3
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Candida albicans biofilms are a major cause of voice prosthesis deterioration in laryngectomized patients. The aim of this study was to produce a surface capable of inhibiting C. albicans biofilm formation. Dimethylaminoethylmethacrylate (DMAEMA) and polyethylenimine (PEI) moieties were covalently bound to the surface of polydimethylsiloxane (PDMS) or polymethylmethacrylate (PMMA) and subsequently quaternized. Physicochemical characterization of the grafted surfaces was carried out and their effect on C. albicans cell numbers was assessed using a modified Robbins device to grow the biofilms. Covalently bound quaternized polyDMAEMA (polyDMAEMAq) and PEI (PEIq) inhibited biofilm growth, with reductions up to 92%. Our approach may show promise for future application in medical devices such as catheters and prostheses.
引用
收藏
页码：213 / 221
页数：9
相关论文
共 41 条
[1]   Colonization of voice prostheses by albicans and non-albicans Candida species [J].
Bauters, TGM ;
Moerman, M ;
Vermeersch, H ;
Nelis, HJ .
LARYNGOSCOPE, 2002, 112 (04) :708-712
[2]   The effect of covalent surface immobilization on the bactericidal efficacy of a quaternary ammonium compound [J].
Brizzolara, Robert A. ;
Stamper, David M. .
SURFACE AND INTERFACE ANALYSIS, 2007, 39 (07) :559-566
[3]   Radiation grafting of N,N-dimethylaminoethylmethacrylate and 4-vinylpyridine onto polypropylene by the one- and two-step methods [J].
Bucio, E ;
Aliev, R ;
Burillo, G .
POLYMER BULLETIN, 2002, 47 (06) :571-577
[4]   Radiation grafting of dimethylaminoethylmethacrylate onto poly(propylene) [J].
Bucio, E ;
Aliev, R ;
Burillo, G .
RADIATION PHYSICS AND CHEMISTRY, 1998, 52 (1-6) :193-196
[5]  
Burillo G, 2000, J APPL POLYM SCI, V78, P972, DOI 10.1002/1097-4628(20001031)78:5<972::AID-APP50>3.0.CO
[6]  
2-1
[7]   Temperature and pH-sensitive swelling behavior of binary DMAEMA/4VP grafts on poly(propylene) films [J].
Burillo, Guillermina ;
Bucio, Emilio ;
Arenas, Ever ;
Lopez, Gabriel P. .
MACROMOLECULAR MATERIALS AND ENGINEERING, 2007, 292 (02) :214-219
[8]   Use of the modified Robbins device to study the in vitro biofilm removal efficacy of NitrAdine™, a novel disinfecting formula for the maintenance of oral medical devices [J].
Coenye, T. ;
De Prijck, K. ;
De Wever, B. ;
Nelis, H. J. .
JOURNAL OF APPLIED MICROBIOLOGY, 2008, 105 (03) :733-740
[9]   Inhibition of Candida albicans Biofilm Formation by Antimycotics Released from Modified Polydimethyl Siloxane [J].
De Prijck, Kristof ;
De Smet, Nele ;
Honraet, Kris ;
Christiaen, Steven ;
Coenye, Tom ;
Schacht, Etienne ;
Nelis, Hans J. .
MYCOPATHOLOGIA, 2010, 169 (03) :167-174
[10]   Candida albicans biofilm formation on peptide functionalized polydimethylsiloxane [J].
De Prijck, Kristof ;
De Smet, Nele ;
Rymarczyk-Machal, Monika ;
Van Driessche, Gonzalez ;
Devreese, Bart ;
Coenye, Tom ;
Schacht, Etienne ;
Nelis, Hans J. .
BIOFOULING, 2010, 26 (03) :269-275
12345