Prevention of Candida albicans Biofilm Formation by Covalently Bound Dimethylaminoethylmethacrylate and Polyethylenimine

被引：37
作者：
De Prijck, Kristof [1 ]
De Smet, Nele [2 ]
Coenye, Tom [1 ]
Schacht, Etienne [2 ]
Nelis, Hans J. [1 ]
机构：
[1] Univ Ghent, Lab Pharmaceut Microbiol, Fac Pharmaceut Sci, B-9000 Ghent, Belgium
[2] Univ Ghent, Lab Polymer Chem & Biomat, B-9000 Ghent, Belgium
来源：
MYCOPATHOLOGIA | 2010年 / 170卷 / 04期
关键词：
Grafting; Polyethylenimine; Dimethylaminoethylmethacrylate; Candida albicans; Biofilm; Modified Robbins Device; BIOLOGICALLY-ACTIVE POLYMERS; AMMONIUM SILANE COATINGS; QUATERNARY AMMONIUM; ANTIMICROBIAL ACTIVITY; SURFACE MODIFICATION; VOICE PROSTHESES; IN-VITRO; REHABILITATION; IMMOBILIZATION; COLONIZATION;
D O I：
10.1007/s11046-010-9316-3
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Candida albicans biofilms are a major cause of voice prosthesis deterioration in laryngectomized patients. The aim of this study was to produce a surface capable of inhibiting C. albicans biofilm formation. Dimethylaminoethylmethacrylate (DMAEMA) and polyethylenimine (PEI) moieties were covalently bound to the surface of polydimethylsiloxane (PDMS) or polymethylmethacrylate (PMMA) and subsequently quaternized. Physicochemical characterization of the grafted surfaces was carried out and their effect on C. albicans cell numbers was assessed using a modified Robbins device to grow the biofilms. Covalently bound quaternized polyDMAEMA (polyDMAEMAq) and PEI (PEIq) inhibited biofilm growth, with reductions up to 92%. Our approach may show promise for future application in medical devices such as catheters and prostheses.
引用
收藏
页码：213 / 221
页数：9
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