Real-time PCR assay for the identification of cutaneous Leishmania parasite species in Constantine region of Algeria

被引：32

作者：

de Monbrison, Frederique ^{[1
]}

Mihoubi, Ilhem

Picot, Stephane

机构：

[1] Hop Edouard Herriot, Serv Parasitol, Hospices Civils Lyon, Lyon, France

[2] Univ Lyon 1, EA 37 32, F-69365 Lyon, France

[3] Univ Mensouri, Lab Biol & Environm, Constantine, Algeria

来源：

ACTA TROPICA | 2007年 / 102卷 / 02期

关键词：

cutaneous leishmaniasis; filter paper; real-time polymerase chain reaction; species identification;

D O I：

10.1016/j.actatropica.2007.04.001

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We evaluated the use of real-time PCR for the identification of cutaneous Leishmania species. The assay, based on the melting curve analysis of fluorescent products, allows the discrimination of four culture adapted strains (L. infantum MHOM/TN/80/IPT1, L. major MHOM/SU/73/5-ASKH, L. donovani MHOM/IN/80/DD8 and L. tropica MHOM/SU/74/K27). One hundred and twenty-nine skin lesions, spotted on filter paper, were collected from patients consulting for suspicion of cutaneous leishmaniasis (CL) at the parasitology laboratory of Constantine Hospital (Algeria). Ninety-seven (75.2%) of the samples analyzed were positive. Sixty-one (5%) were related to L. major strain. These results indicate that PCR assay provides pleasant results with filter paper and represents a tool for the identification of old world CL. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：79 / 83

页数：5

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