Analytical Ultracentrifugation and Its Role in Development and Research of Therapeutical Proteins

被引：19

作者：

Liu, Jun ^{[1
]}

Yadav, Sandeep ^{[1
]}

Andya, James ^{[1
]}

Demeule, Barthelemy ^{[1
]}

Shire, Steven J. ^{[1
]}

机构：

[1] Genentech Inc, Late Stage Pharamaceut & Proc Dev, San Francisco, CA 94080 USA

来源：

ANALYTICAL ULTRACENTRIFUGATION | 2015年 / 562卷

关键词：

PARTIAL SPECIFIC VOLUMES; SIZE-EXCLUSION CHROMATOGRAPHY; ENDOTHELIAL GROWTH-FACTOR; SELF-ASSOCIATION; MONOCLONAL-ANTIBODIES; MOLECULAR-WEIGHT; SEDIMENTATION EQUILIBRIUM; ESCHERICHIA-COLI; HUMAN RELAXIN; TISSUE FACTOR;

D O I：

10.1016/bs.mie.2015.04.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The historical contributions of analytical ultracentrifugation (AUC) to modern biology and biotechnology drug development and research are discussed. AUC developed by Svedberg was used to show that proteins are actually large defined molecular entities and also provided the first experimental verification for the semiconservative replication model for DNA initially proposed by Watson and Crick. This chapter reviews the use of AUC to investigate molecular weight of recombinant-DNA-produced proteins, complex formation of antibodies, intermolecular interactions in dilute and high concentration protein solution, and their impact on physical properties such as solution viscosity. Recent studies using a "competitive binding" analysis by AUC have been useful in critically evaluating the design and interpretation of surface plasmon resonance measurements and are discussed. The future of this technology is also discussed including prospects for a new higher precision analytical ultracentrifuge.

引用

页码：441 / 476

页数：36

共 31 条

[31] Genome-wide identification and comparative in-silico characterization of β-galactosidase (GH-35) in ascomycetes and its role in germ tube development of Aspergillus fumigatus via RNA-seq analysis [J].

Baig, Danish Ilyas ;

Zafar, Zeeshan ;

Khan, Haris Ahmed ;

Younus, Amna ;

Bhatti, Muhammad Faraz .

PLOS ONE, 2023, 18 (06)

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