Manure application effects on subsoils: Abundant taxa initiate the diversity reduction of rare bacteria and community functional alterations

被引:35
作者
Liu, Xipeng [1 ,2 ,3 ]
Wang, Haotian [4 ]
Wu, Yingjie [5 ]
Bi, Qingfang [6 ]
Ding, Kai [2 ]
Lin, Xianyong [1 ]
机构
[1] Zhejiang Univ, Coll Environm & Resource Sci, Zhejiang Prov Key Lab Agr Resources & Environm, Hangzhou 310058, Peoples R China
[2] Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, 1799 Jimei Rd, Xiamen 361021, Peoples R China
[3] Univ Groningen, Groningen Inst Evolutionary Life Sci GELIFES, Microbial Ecol Cluster, Genom Res Ecol & Evolut Nat GREEN, NL-9747AG Groningen, Netherlands
[4] Thunen Inst Biodivers, D-38116 Braunschweig, Germany
[5] Sichuan Agr Univ, Coll Resources, Chengdu 611130, Peoples R China
[6] Max Planck Inst Biogeochem, D-07745 Jena, Germany
基金
中国国家自然科学基金;
关键词
Excessive manure application; Rare biosphere; HT-qPCR; Deep soil; Microbial carbon mineralization; SOIL MICROBIAL COMMUNITY; ORGANIC-MATTER; LABILE CARBON; RIBOSOMAL-RNA; LAND-USE; BIOMASS; FERTILIZER; NITROGEN; QUALITY; MINERALIZATION;
D O I
10.1016/j.soilbio.2022.108816
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
Manure application is a significant agriculture practice that can affect the microbial community structure in both topsoil and subsoil. However, how the abundant and rare taxa in the soil profile respond to surface manure application and their ecological roles at different soil depths remain unknown. Based on a soil mesocosm experiment, we used amplicon sequencing and high-throughput quantitative-PCR array to characterize the bacterial community composition and functional traits across soil profiles. Results showed that the relative abundance and diversity of rare taxa increased in the topsoil but decreased in the 40-60 cm soil, where the relative abundance of abundant taxa increased after manure application. Evidence from network analysis, niche width and 16S rRNA operon (rrn) copy number suggest that abundant taxa in subsoils had a greater competitivity for resource mining than rare taxa. Regarding resource utilization strategies, microbial communities under manure treatment preferred to utilize recalcitrant carbon (C) and inorganic phosphorus (P) in the topsoil and labile carbon and organic phosphorus in the subsoil compared with non-manure application. Random Forest regression analysis indicated the relatively greater contribution of abundant taxa to C degradation and fixation in subsoils. These results imply that the manure application effect on microbial resource mining in subsoils is mainly driven by abundant bacteria due to the lower competitiveness of rare taxa for the C sources leached above. Overall, This study linked community functions with the ecological role of abundant and rare taxa in the context of environmental disturbances at different soil depths and shed light on the potential ecological risks of diversity reduction of subsoil rare bacteria.
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页数:10
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