Sirt1 exerts anti-inflammatory effects and promotes steroidogenesis in Leydig cells

被引：28

作者：

Wu, Ling ^{[1
]}

Zhang, Aijun ^{[1
]}

Sun, Yijuan ^{[1
]}

Zhu, Xiaobin ^{[1
]}

Fan, Weimin ^{[1
]}

Lu, Xiaowei ^{[1
]}

Yang, Qingqing ^{[1
]}

Feng, Yun ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Ruijin Hosp, Reprod Med Ctr, Sch Med, Shanghai 200025, Peoples R China

来源：

FERTILITY AND STERILITY | 2012年 / 98卷 / 01期

关键词：

Leydig cells; steroidogenesis; Sirt1; SF-1; proinflammatory cytokines; TUMOR-NECROSIS-FACTOR; ALPHA;

D O I：

10.1016/j.fertnstert.2012.04.008

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To investigate the anti-inflammatory effects of Sirt1 and its roles in steroidogenesis in Leydig cells. Design: In vitro cell culture study. Setting: Reproductive medical center. Animal(s): C57BL/6 male mice. Intervention(s): TM3 Leydig cells were treated with proinflammatory cytokines including tumor necrosis factor-alpha, interleukin-6, or interleukin-1 beta. Main Outcomes Measure(s): Sirt1 mRNA and protein levels were measured by real-time polymerase chain reaction and Western blotting in TM3 Leydig cells treated with proinflammatory cytokines. The cell viability was determined using MTT and BrdU incorporation assays. The effect of Sirt1 on the coactivation of steroidogenic factor 1 (SF-1) was characterized by coimmunoprecipitation and luciferase reporter assays. Result(s): Sirt1 mRNA and protein levels were significantly down-regulated by proinflammatory cytokines treatment in TM3 Leydig cells. Sirt1 agonist or overexpression could efficiently protect cytotoxicity induced by proinflammatory cytokines. Sirt1 promoted steroidogenesis through its coactivation of SF-1. Conclusion(s): Sirt1 plays protective roles and promotes steroidogenesis in Leydig cells through its anti-inflammatory actions and coactivation of SF-1. (Fertil Steril (R) 2012; 98: 194-9. (C) 2012 by American Society for Reproductive Medicine.)

引用

页码：194 / 199

页数：6

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