Evaluation of a rapid assay for identification of Mycobacterium tuberculosis grown in solid and liquid media

被引:10
作者
Ang, C. F. [3 ]
Cajucom, M. A. M. [3 ]
Kim, Y. [4 ]
Bang, H. [4 ]
Lee, H. [4 ]
Cho, S. N. [1 ,2 ]
Montalban, C. S. [3 ]
机构
[1] Yonsei Univ, Coll Med, Dept Microbiol, Seoul 120752, South Korea
[2] Yonsei Univ, Coll Med, Inst Immunol & Immunol Dis, Seoul 120752, South Korea
[3] Univ Philippines, Philippine Gen Hosp, Dept Med, Infect Dis Sect, Manila, Philippines
[4] Yonsei Univ, Coll Hlth Sci, Dept Biomed Lab Sci, Wonju, South Korea
关键词
identification; MPT64; antigen; Mycobacterium tuberculosis; CAPILIA TB ASSAY; CULTURE CONFIRMATION; COMPLEX; DIAGNOSIS; KIT;
D O I
10.5588/ijtld.10.0709
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
An MPT64 antigen detection assay, the SD Bioline TB Ag MPT64 Rapid kit, was evaluated for the identification of Mycobacterium tuberculosis grown in both solid and liquid media. The rapid test showed positive results in 132 of 133 (99.2%) M. tuberculosis cultures grown on Ogawa medium and 136/143 (95.1%) cultures grown in liquid culture medium, resulting in an overall sensitivity of 97.1%. All 18 non-tuberculous mycobacteria were found to be negative by the rapid test, indicating a specificity of 100%. The rapid test seems useful for the initial confirmation of M. tuberculosis in acid-fast bacilli positive cultures.
引用
收藏
页码:1475 / 1477
页数:3
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