Gold nanoparticle-assisted delivery of brain-derived neurotrophic factor to cerebral organoids

被引：2

作者：

Park, Sung Bum ^{[1
]}

Cho, Hye-Jin ^{[2
]}

Moon, Sang Rin ^{[1
]}

Choi, Kyoung Jin ^{[1
]}

Jung, Won Hoon ^{[1
]}

Kim, Ki Young ^{[1
]}

Koh, Byumseok ^{[1
]}

机构：

[1] Korea Res Inst Chem Technol, Therapeut & Biotechnol Div, 141 Gajeong Ro, Daejeon 34114, South Korea

[2] Korea Res Inst Chem Technol, Chem Platform Technol Div, 141 Gajeong Ro, Daejeon 34114, South Korea

来源：

NANO RESEARCH | 2022年 / 15卷 / 04期

关键词：

gold nanoparticles; cerebral organoids; delivery; penetration; differentiation; viability; DRUG-DELIVERY; ASTROCYTES; GENERATION; NEURONS; CARRIER; MODELS;

D O I：

10.1007/s12274-021-3975-x

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Gold nanoparticle (AuNP)-assisted delivery of proteins/drugs to cells or in vivo systems has long been studied. However, their interaction with in vivo mimicking organoids is still largely unknown. Here, we conjugated brain-derived neurotropic factor (BDNF) to the AuNP surface and monitored how AuNP-BDNF interacts with cerebral organoids. Data suggest that AuNP-BDNF can successfully enter cerebral organoids and can be found up to 15.21 pm inside cerebral organoids. AuNP-BDNF does not induce significant cytotoxicity to cerebral organoids at concentrations up to 2 nM and increases the expression of 75 genes compared to control cerebral organoids. These data suggest the successful delivery of BDNF through AuNP-BDNF conjugation in cerebral organoids, and the conjugation can affect its differentiation.

引用

页码：3099 / 3105

页数：7

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