Antiviral Effect of Selenomethionine on Porcine Deltacoronavirus in Pig Kidney Epithelial Cells

被引：11

作者：

Ren, Zhihua ^{[1
]}

Jia, Guilin ^{[1
]}

He, Hongyi ^{[1
]}

Ding, Ting ^{[1
]}

Yu, Yueru ^{[1
]}

Zuo, ZhiCai ^{[1
]}

Hu, Yanchun ^{[1
]}

Zhong, Zhijun ^{[1
]}

Yu, Shumin ^{[1
]}

Deng, Huidan ^{[1
]}

Shen, Liuhong ^{[1
]}

Cao, Suizhong ^{[1
]}

Peng, Guangneng ^{[1
]}

Wang, Ya ^{[1
]}

Cai, Dongjie ^{[1
]}

Gou, Liping ^{[1
]}

Ma, Xiaoping ^{[1
]}

Liu, Haifeng ^{[1
]}

Zhou, Ziyao ^{[1
]}

Deng, Youtian ^{[1
]}

Yang, Dingyong ^{[2
]}

Deng, Junliang ^{[1
]}

机构：

[1] Sichuan Agr Univ, Coll Vet Med, Key Lab Anim Dis & Human Hlth Sichuan Prov, Chengdu, Peoples R China

[2] Chengdu Agr Coll, Coll Anim Husb & Vet Med, Chengdu, Peoples R China

来源：

FRONTIERS IN MICROBIOLOGY | 2022年 / 13卷

关键词：

porcine deltacoronavirus; antiviral activities; antioxidant; selenomethionine; innate immunity; EPIDEMIC DIARRHEA VIRUS; SELENIUM; METABOLISM; IMMUNITY; LIVER;

D O I：

10.3389/fmicb.2022.846747

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Porcine deltacoronavirus (PDCoV) is an emerging porcine intestinal coronavirus in recent years, which mainly causes different degrees of vomiting and diarrhea in piglets and has caused great harm to the swine husbandry worldwide since its report. Selenium is an essential trace element for organisms and has been demonstrated to have antiviral effects. In this study, pig kidney epithelial (LLC-PK) cells were used to study the antiviral activity of selenomethionine (Se-Met) (2, 4, 8, and 16 mu M) against PDCoV by detecting the replication of the virus, the expression of the mitochondrial antiviral signal protein (MAVS) protein, and the phosphorylation of interferon regulatory factor-3 (IRF-3), IFN-alpha, and IFN-beta, and the changes in glutathione content, glutathione peroxidase, superoxide dismutase activity, and hydrogen peroxide content in the cells. The results showed that Se-Met at higher than physiological concentrations (16 mu M) could significantly inhibit the replication of PDCoV in LLC-PK cells and enhance the expression of MAVS protein and the phosphorylation of IRF-3. In addition, Se-Met also improved the intracellular production of IFN alpha/beta and antioxidant capacity with increasing doses. These data suggest that the availability of selenium through selenomethionine supports the antiviral response in porcine kidney cells, and the specific mechanism is attributed to the improved cellular antioxidant capacity and activation of the MAVS pathway by Se-Met.

引用

页数：6

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