A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens

被引：22

作者：

Kearns, AM

Freeman, R

Steward, M

Magee, JG

机构：

[1] Gen Hosp, Publ Hlth Lab, Newcastle Upon Tyne, Tyne & Wear, England

[2] Novocastra Labs Ltd, Newcastle Upon Tyne, Tyne & Wear, England

来源：

JOURNAL OF CLINICAL PATHOLOGY | 1998年 / 51卷 / 12期

关键词：

polymerase chain reaction; Mycobacterium tuberculosis;

D O I：

10.1136/jcp.51.12.922

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

A rapid in-house polymerase chain reaction (PCR) assay is described for the direct detection of Mycobacterium tuberculosis complex in clinical material. Its performance is compared with two kit based systems. The results of the in-house assay were comparable with the commercial assays, detecting M tuberculosis in 100% of smear positive, culture positive samples. The in-house assay proved to be rapid, easy, and inexpensive to pet-form, and the inclusion of an internal inhibitor control permitted validation of the PCR results.

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收藏

页码：922 / 924

页数：3

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