Arabidopsis MKK4 mediates osmotic-stress response via its regulation of MPK3 activity

被引：102
|
作者：
Kim, Sun-Ho [1 ]
Woo, Dong-Hyuk [1 ]
Kim, Jae-Min [1 ]
Lee, Sun-Young [1 ]
Chung, Woo Sik [2 ]
Moon, Yong-Hwan [1 ]
机构：
[1] Pusan Natl Univ, Dept Mol Biol, Pusan 609735, South Korea
[2] Gyeongsang Natl Univ, Plant Mol Biol & Biotechnol Res Ctr, Div Appl Life Sci, Program BK21, Jinju 660701, South Korea
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 412卷 / 01期
关键词：
Arabidopsis; MKK4; MPK3; Osmotic stress; NCED3; RD29A; ACTIVATED PROTEIN-KINASES; MAP KINASE; SACCHAROMYCES-CEREVISIAE; THALIANA; PLANTS; CASCADE; ACID; TRANSFORMATION; EXPRESSION; TOLERANCE;
D O I：
10.1016/j.bbrc.2011.07.064
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Plants have developed disparate regulatory pathways to adapt to environmental stresses. In this study, we identified MKK4 as an important mediator of plant response to osmotic stress. mkk4 mutants were more sensitive to high salt concentration than WT plants, exhibiting higher water-loss rates under dehydration conditions and additionally accumulating high levels of ROS. In contrast, MKK4-overexpressing transgenic plants showed tolerance to high salt as well as lower water-loss rates under dehydration conditions. In-gel kinase assays revealed that MKK4 regulates the activity of MPK3 upon NaCl exposure. Semi-quantitative RT-PCR analysis showed that expression of NCED3 and RD29A was lower and higher in mkk4 mutants and MKK4-overexpressing transgenic plants, respectively. Taken together, our results suggest that MKK4 is involved in the osmotic-stress response via its regulation of MPK3 activity. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：150 / 154
页数：5
相关论文
共 35 条
  • [21] The MKK3-MPK7 cascade phosphorylates ERF4 and promotes its rapid degradation to release seed dormancy in Arabidopsis
    Chen, Xi
    Li, Qiujia
    Ding, Ling
    Zhang, Shengnan
    Shan, Siyao
    Xiong, Xiong
    Jiang, Wenhui
    Zhao, Bo
    Zhang, Liying
    Luo, Ying
    Lian, Yiming
    Kong, Xiuqin
    Ding, Xiali
    Zhang, Jun
    Li, Chunli
    Soppe, Wim J. J.
    Xiang, Yong
    MOLECULAR PLANT, 2023, 16 (11) : 1743 - 1758
  • [22] H2S Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in Arabidopsis
    Du, Xinzhe
    Jin, Zhuping
    Liu, Zhiqiang
    Liu, Danmei
    Zhang, Liping
    Ma, Xiaoli
    Yang, Guangdong
    Liu, Sha
    Guo, Yarong
    Pei, Yanxi
    FRONTIERS IN MOLECULAR BIOSCIENCES, 2021, 8
  • [23] Involvement of MPK4 in osmotic stress response pathways in cell suspensions and plantlets of Arabidopsis thaliana:: activation by hypoosmolarity and negative role in hyperosmolarity tolerance
    Droillard, MJ
    Boudsocq, M
    Barbier-Brygoo, H
    Laurière, C
    FEBS LETTERS, 2004, 574 (1-3): : 42 - 48
  • [24] Sequence and functional analyses of the aldehyde dehydrogenase 7B4 gene promoter in Arabidopsis thaliana and selected Brassicaceae: regulation patterns in response to wounding and osmotic stress
    Tagnon D. Missihoun
    Quancan Hou
    Daniela Mertens
    Dorothea Bartels
    Planta, 2014, 239 : 1281 - 1298
  • [25] Sequence and functional analyses of the aldehyde dehydrogenase 7B4 gene promoter in Arabidopsis thaliana and selected Brassicaceae: regulation patterns in response to wounding and osmotic stress
    Missihoun, Tagnon D.
    Hou, Quancan
    Mertens, Daniela
    Bartels, Dorothea
    PLANTA, 2014, 239 (06) : 1281 - 1298
  • [26] Ethylene Response Factor LlERF110 Mediates Heat Stress Response via Regulation of LlHsfA3A Expression and Interaction with LlHsfA2 in Lilies (Lilium longiflorum)
    Wang, Yue
    Zhou, Yunzhuan
    Wang, Rui
    Xu, Fuxiang
    Tong, Shi
    Song, Cunxu
    Shao, Yanan
    Yi, Mingfang
    He, Junna
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (24)
  • [27] GPD1, WHICH ENCODES GLYCEROL-3-PHOSPHATE DEHYDROGENASE, IS ESSENTIAL FOR GROWTH UNDER OSMOTIC-STRESS IN SACCHAROMYCES-CEREVISIAE, AND ITS EXPRESSION IS REGULATED BY THE HIGH-OSMOLARITY GLYCEROL RESPONSE PATHWAY
    ALBERTYN, J
    HOHMANN, S
    THEVELEIN, JM
    PRIOR, BA
    MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (06) : 4135 - 4144
  • [28] Unique regulation of glyoxalase I activity during osmotic stress response in the fission yeast Schizosaccharomyces pombe: neither the mRNA nor the protein level of glyoxalase I increase under conditions that enhance its activity
    Yoshifumi Takatsume
    Shingo Izawa
    Yoshiharu Inoue
    Archives of Microbiology, 2005, 183 : 224 - 227
  • [29] Unique regulation of glyoxalase I activity during osmotic stress response in the fission yeast Schizosaccharomyces pombe:: neither the mRNA nor the protein level of glyoxalase I increase under conditions that enhance its activity
    Takatsume, Y
    Izawa, S
    Inoue, Y
    ARCHIVES OF MICROBIOLOGY, 2005, 183 (03) : 224 - 227
  • [30] Rho5p is involved in mediating the osmotic stress response in Saccharomyces cerevisiae, and its activity is regulated via Msi1p and Npr1p by phosphorylation and ubiquitination
    Annan, Robert B.
    Wu, Cunle
    Waller, Daniel D.
    Whiteway, Malcolm
    Thomas, David Y.
    EUKARYOTIC CELL, 2008, 7 (09) : 1441 - 1449
1234