Crystallization and preliminary X-ray crystallographic analysis of the Hsp100 chaperone ClpB from Thermus thermophilus

被引：6
|
作者：
Lee, S
Hisayoshi, M
Yoshida, M
Tsai, FTF [1 ]
机构：
[1] Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Houston, TX 77030 USA
[2] Tokyo Inst Technol, Chem Resources Lab, Yokohama, Kanagawa 2268503, Japan
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2003年 / 59卷
关键词：
D O I：
10.1107/S0907444903023266
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
ClpB from Thermus thermophilus (TClpB) has been crystallized by the vapour-diffusion method in the presence of adenosine 5'-(beta,gamma-imido) triphosphate (AMPPNP) and adenosine 5'-(gamma-thio) triphosphate (ATPgammaS), respectively. Complete native data sets have been collected from frozen crystals, which belonged to the primitive orthorhombic space group P2(1)2(1)2(1) with unit-cell parameters a = 109.2, b = 139.6, c = 213.0 Angstrom, alpha = beta = gamma = 90degrees.
引用
收藏
页码：2334 / 2336
页数：3
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