Effect of Conditioned Media of Limbal Epithelial Cells and Corneal Stromal Cells on Functional Activity of Limb Mesenchymal Stem Cells

被引：0

作者：

Bondarenko, N. A. ^{[1
]}

Surovtseva, M. A. ^{[1
]}

Kim, I. I. ^{[1
]}

Lykov, A. P.

Iskakov, I. A. ^{[2
]}

Trunov, A. N. ^{[2
]}

Chernykh, V. V. ^{[2
]}

Poveshchenko, O. V. ^{[1
]}

机构：

[1] Russian Acad Sci, Res Inst Clin & Expt Lymphol, Siberian Div, Affiliated Branch,Fed Res Ctr,Inst Cytol & Genet, Novosibirsk, Russia

[2] Russian Minist Hlth, SN Fyodorov Eye Microsurg Fed State Inst, Novosibirsk Branch, Novosibirsk, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2022年 / 173卷 / 04期

关键词：

mesenchymal stem cells; limbal epithelial stem cells; conditioned media; keratocytes; corneal fibroblasts;

D O I：

10.1007/s10517-022-05562-8

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We studied the effect of conditioned media from limbal epithelial stem cells, fibroblasts, and corneal keratocytes on the functional activity of human limbal mesenchymal stem cells. It was shown that the conditioned media from limbal epithelial stem cells reduced proliferative activity and inhibited migration of limbal mesenchymal stem cells. In the conditioned media of limbal epithelial stem cells, increased concentrations of VEGF and TNF alpha and reduced concentration of BDNF, vimentin, and fibronectin were found. The conditioned medium from corneal stromal cells did not affect functional activity of mesenchymal stem cells in the limbus. These data contribute to the understanding of the interaction of cells in the limbal niche and with corneal cells essential for the maintenance of the cellular homeostasis in the cornea.

引用

页码：464 / 467

页数：4

共 14 条

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