Detection of Salmonella DNA in chicken embryos and environmental samples by polymerase chain reaction

被引：7
|
作者：
Tuchili, LM
Kodama, H
Sharma, RN
Takatori, I
Pandey, GS
Kabilika, S
Mukamoto, M
Tsuji, S
Baba, T
机构：
[1] UNIV OSAKA PREFECTURE,COLL AGR,DEPT VET SCI,LAB VET IMMUNOL,SAKAI,OSAKA 593,JAPAN
[2] UNIV ZAMBIA,SCH VET MED,LUSAKA,ZAMBIA
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 1996年 / 58卷 / 09期
关键词：
chicken embryo; DNA detection; polymerase chain reaction; Salmonella;
D O I：
10.1292/jvms.58.881
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
By polymerase chain reaction (PCR) using a pair of primers specific for Salmonella phoE: gene a 365-bp specific gene fragment could be amplified from yolk of infertile eggs and dead-in-shell chicken embryos, and from environmental samples. Out of 45 dead-in-shell embryo samples, 20 (44.4%) were found positive for Salmonella DNA by PCR compared to 11 (24.4%) by bacteria isolation. Salmonella DNA could also be detected from infertile eggs, chicken farces, floor litter and chick fluff, which incidence was higher than that by bacteria isolation.
引用
收藏
页码：881 / 884
页数：4
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