Simple and rapid detection of Salmonella sp. from cattle feces using polymerase chain reaction (PCR) in Iran

The aim of this study was to employ biochemical and molecular assays to detect and diagnose Salmonella in cattle. For this reason, 1124 fecal samples were collected from cattle in Markazi provinces of Iran. Selective specific culture media for Salmonella were used to grow a number of isolates from the cattle samples. Salmonella bacteria were identified with biochemical test. The antimicrobial susceptibility test with disc diffusion method was performed on samples of Salmonella by using a molecular based approach, and it was possible to identify Salmonella sp by amplifying specific genes ''16s rRNA'' as a step for identification. Our studies showed that the molecular-based approach are more rapid for initial detection of Salmonella SP.