Activation of chloride secretion via proteinase-activated receptor 2 in a human eccrine sweat gland cell line - NCL-SG3

被引：15

作者：

Bovell, Douglas L. ^{[2
]}

Santic, Radmila ^{[3
]}

Kofler, Barbara ^{[3
]}

Hermann, Anton ^{[4
]}

Wilson, Darius ^{[2
]}

Corbett, Alistair ^{[2
]}

Lang, Roland ^{[1
]}

机构：

[1] Paracelsus Med Univ Salzburg, Univ Hosp Salzburg, Dept Dermatol, A-5020 Salzburg, Austria

[2] Glasgow Caledonian Univ, Dept Biol & Biomed Sci, Glasgow G4 0BA, Lanark, Scotland

[3] Salzburg Univ, Dept Pediat, A-5020 Salzburg, Austria

[4] Salzburg Univ, Dept Cell Biol, A-5020 Salzburg, Austria

来源：

EXPERIMENTAL DERMATOLOGY | 2008年 / 17卷 / 06期

关键词：

eccrine sweat gland cells; ion transport; NCL-SG3; cells; PAR-2; proteinase-activated receptors;

D O I：

10.1111/j.1600-0625.2007.00659.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Proteinase-activated receptor 2 (PAR-2) has been shown to elicit secretion in a variety of secretory epithelial cells by the transepithelial movement of chloride ions across the apical membrane. However, it is not known whether these receptors are present and/or functional in the secretory epithelial cells of the human eccrine sweat gland. To investigate this possibility mRNA analysis, Ca2+ microspectrofluorimetry and the short circuit current (Isc) technique were used to quantify electrolyte transport in a cell line (NCL-SG3) derived from human eccrine sweat gland secretory epithelia. The results provided molecular and functional evidence of the presence of PAR-2 receptors in the NCL-SG3 cells and show that these receptors can activate transepithelial Cl- secretion possibly via Ca2+-activated Cl- channels.

引用

页码：505 / 511

页数：7

共 51 条

[1] Basolateral proteinase-activated receptor (PAR-2) induces chloride secretion in M-1 mouse renal cortical collecting duct cells [J].

Bertog, M ;

Letz, B ;

Kong, WY ;

Steinhoff, M ;

Higgins, MA ;

Bielfeld-Ackermann, A ;

F'römter, E ;

Bunnett, NW ;

Korbmacher, C .

JOURNAL OF PHYSIOLOGY-LONDON, 1999, 521 (01) :3-17

[2]

Bohm SK, 1996, BIOCHEM J, V314, P1009

[3] The absence of apoeccrine glands in the human axilla has disease pathogenetic implications, including axillary hyperhidrosis [J].

Bovell, D. L. ;

Corbett, A. D. ;

Holmes, S. ;

MacDonald, A. ;

Harker, M. .

BRITISH JOURNAL OF DERMATOLOGY, 2007, 156 (06) :1278-1286

[4]

Bovell DL, 2002, J PHYSIOL-LONDON, V544, p107P

[5] ION-TRANSPORT IN CULTURED EPITHELIA FROM HUMAN SWEAT GLANDS - COMPARISON OF NORMAL AND CYSTIC-FIBROSIS TISSUES [J].

BRAYDEN, DJ ;

PICKLES, RJ ;

CUTHBERT, AW .

BRITISH JOURNAL OF PHARMACOLOGY, 1991, 102 (01) :57-64

[6] A protective role for protease-activated receptors in the airways [J].

Cocks, TM ;

Fong, B ;

Chow, JM ;

Anderson, GP ;

Frauman, AG ;

Goldie, RG ;

Henry, PJ ;

Carr, MJ ;

Hamilton, JR ;

Moffatt, JD .

NATURE, 1999, 398 (6723) :156-160

[7] Basolateral PAR-2 receptors mediate KCl secretion and inhibition of Na+ absorption in the mouse distal colon [J].

Cuffe, JE ;

Bertog, M ;

Velázquez-Rocha, S ;

Dery, O ;

Bunnett, N ;

Korbmacher, C .

JOURNAL OF PHYSIOLOGY-LONDON, 2002, 539 (01) :209-222

[8] KININ EFFECTS ON ELECTROGENIC ION-TRANSPORT IN PRIMARY CULTURES OF PIG RENAL PAPILLARY COLLECTING TUBULE CELLS [J].

CUTHBERT, AW ;

GEORGE, AM ;

MACVINISH, L .

AMERICAN JOURNAL OF PHYSIOLOGY, 1985, 249 (03) :F439-F447

[9]

D'Andrea MR, 1998, J HISTOCHEM CYTOCHEM, V46, P157

[10] Proteinase-activated receptors:: novel mechanisms of signaling by serine proteases [J].

Déry, O ;

Corvera, CU ;

Steinhoff, M ;

Bunnett, NW .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1998, 274 (06) :C1429-C1452

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