Identification of Arcanobacterium hippocoleae by MALDI-TOF MS analysis and by various genotypical properties

被引:10
作者
Wickhorst, J. -P. [1 ]
Sammra, O. [1 ]
Hassan, A. A. [2 ]
Alssashen, M. [1 ]
Laemmler, C. [1 ]
Prenger-Berninghoff, E. [3 ]
Erhard, M. [4 ]
Metzner, M. [4 ]
Paschertz, K. [5 ]
Timke, M. [6 ]
Abdulmawjood, A. [7 ]
机构
[1] Justus Liebig Univ Giessen, Inst Pharmakol & Toxikol, Schubertstr 81, D-35392 Giessen, Germany
[2] Univ Mosul, Dept Vet Publ Hlth, Coll Vet Med, Mosul, Iraq
[3] Justus Liebig Univ Giessen, Inst Hyg & Infekt Krankheiten Tiere, Frankfurterstr 85-91, D-35392 Giessen, Germany
[4] RIPAC LABOR GmbH, Muhlenberg 11, D-14476 Potsdam, Germany
[5] Tierarztpraxis Dorfteich, Dorfteich 8, D-49661 Cloppenburg Bethen, Germany
[6] Bruker Daltonik GmbH, Entwicklung Bioanal, Fahrenheitstr 4, D-28359 Bremen, Germany
[7] Univ Vet Med Hannover, Inst Lebensmittelqualitat & Sicherheit, Bischofsholer Damm 15, D-30173 Hannover, Germany
关键词
Arcanobacterium hippocoleae; MALDI-TOF MS; 16S rRNA gene; 16S-23S rRNA intergenic spacer region; rpoB; tuf; gap; GENUS ARCANOBACTERIUM; SP-NOV; EMENDED DESCRIPTION; HAEMOLYTICUM; BACTERIA; TRUEPERELLA; DOG;
D O I
10.1016/j.rvsc.2017.01.006
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In the present study an Arcanobacterium hippocoleae strain isolated from a uterus swab of an apparently healthy mare could be identified by phenotypic properties, by MALDI-TOF MS analysis and genotypically by investigating the molecular targets 16S rDNA, 16S-23S rDNA intergenic spacer region and the genes encoding the (3 subunit of bacterial RNA polymerase (rpoB), elongation factor tu (tuf) and glyceraldehyde 3-phosphate dehydrogenase (gap). The presented data are one of the few reports about the species A. hippocoleae and might help to elucidate the role this species plays in infections of horses. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码:10 / 12
页数:3
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